We narrowed to 18,356 results for: Tec
-
-
nCas9-SpRY_P2A-mCherry
Plasmid#219935PurposeMammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-SpRY_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABEmax
Plasmid#119808PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)
Plasmid#191180PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s3:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR
Plasmid#118470PurposecpBFP-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertblueAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)
Plasmid#191144PurposeExpression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp longDepositorInsertmCherry:Bdnf.s1:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ
Plasmid#153036PurposeFor CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GWF058
Plasmid#133609PurposeUAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPRDepositorInsertGal4DBD-NS3a, DNCR2-VPR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WAVE 2
Plasmid#67098PurposeStandard for cell-free expression benchmarking.DepositorInsertWAVE 2 (WASF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#138511PurposeP2DepositorInsertinteinC-dSpCas9-UGI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-mSA*
Plasmid#182952PurposeExpress SpCas9-mSA*DepositorInsertSpCas9-mSA*
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
VRER-ABEmax
Plasmid#119812PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (VRER)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58
Plasmid#171432PurposeExpression of hH3-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191182PurposeExpression of EACTB sensor in mammalian cellsDepositorInsertmCherry:ACTB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRRH
Plasmid#136924PurposeExpresses Cas9-NRRH in mammalian cellsDepositorInsertCas9-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT462
Plasmid#139393PurposeXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmRDepositorInsertXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs
UseSynthetic BiologyTagsExpressionMutationPromoteraTC and BA inducible promotersAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSplit-CO-hDib1
Plasmid#58788PurposeExpress LucC-hDib1 fusion protein in mammalian cellsDepositorInserthDib1 (TXNL4A Human)
UseTagsFlag and Luciferase (394-550aa)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
p35S-A3A-PBE
Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CP1041-CBEmax
Plasmid#119803PurposeC-to-T base editorDepositorInsertrApobec1-nSpCas9 (CP1041) -UGI-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Cas9-NRTH
Plasmid#136925PurposeExpresses Cas9-NRTH in mammalian cellsDepositorInsertCas9-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH607-Tier1-PCREm4-Nluc
Plasmid#169594PurposeTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
UseTagsExpressionMammalianMutationPromoterPCREm4Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2365-Tier1-PhCMV-TetR
Plasmid#169566PurposeTier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).DepositorInsertPCMV-driven tetracycline repressor protein class B from transposon Tn10
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
UseTags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CP1249-ABEmax
Plasmid#119809PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF297
Plasmid#133612PurposeTET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFPDepositorInsertTET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-Sp dCas9-mVenusN
Plasmid#191396PurposeSpdCas9 fused to N-terminal part of mVenusDepositorInsertN-terminal part of split mVenus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro NFS1
Plasmid#102977PurposeExpression of NFS1DepositorInsertNFS1 (NFS1 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations to eliminate shNFS1_1 binding si…PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
YSBE 508 : pNEO_GFP
Plasmid#92081PurposePlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selectionDepositorInsertGFP
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSPlit-OC-hDib1
Plasmid#58789PurposeExpress hDib1-LucC fusion protein in mammalian cellsDepositorInserthDib1 (TXNL4A Human)
UseTagsFlag and Luciferase (394-550aa)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1-14-mVenusC
Plasmid#191393PurposeMBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)ExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT tetramutant for Split-PE (pEH101)
Plasmid#190109PurposeExpresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK312
Plasmid#59569PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP ochre attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC154
Plasmid#118902Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
UseTagsTwinStrep, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB
Plasmid#171427PurposeExpression of HaloTag N58-(GGS)X6-FRB in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
CP1012-ABEmax
Plasmid#119806PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU3-gRNA
Plasmid#53063PurposeTranscription of guide RNA (gRNA) in rice cellsDepositorInsertOsU3 promoter + gRNA scaffold
UseCRISPR; Plant expressionTagsExpressionMutationPromoterrice U3Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluB-NP
Plasmid#165093Purposeprotein expression in E. coliDepositorInsertInfluenza B virus(B/Georgia/05/2019) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCoofy48
Plasmid#55186PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…ExpressionBacterialMutationPromoterT7Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC153
Plasmid#118901Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsTwinStrep, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only
