We narrowed to 4,881 results for: phy
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Plasmid#22214DepositorInsertTuba (DNMBP Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-ER
Plasmid#125121PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-ER
UseTagsER retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SUMO1
Plasmid#185637Purposeexpression of GST-tagged SUMO1 in bacterial cellsDepositorInsertsmall ubiquitin-like modifier 1 (SUMO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ERT2-iCre-ERT2
Plasmid#178059PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.DepositorInsertERT2-iCre-ERT2
UseAAVTagsExpressionMammalianMutationPromoterHuman synapsin 1 (Syn) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K20-P2A-mCherry
Plasmid#185618PurposeRibosomal stalling reporter: positive controlDepositorInsertGFP-K20(AAA)-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K0-P2A-mCherry
Plasmid#185619PurposeRibosomal stalling reporter: emptyDepositorInsertGFP-empty-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-I15-P2A-mCherry
Plasmid#185620PurposeRibosomal stalling reporter: 15x isoleucineDepositorInsertGFP-I15-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-T2TP
Plasmid#114725Purposeexpression in mammalian cellsDepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQ
Plasmid#166948PurposeLentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationPromotereF1aAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2
Plasmid#194680PurposeCMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2DepositorInsertHpep1-betaArrestin2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GLS-3xFLAG
Plasmid#163450Purposelentiviral overexpression vector for human GLSDepositorInsertGLS (GLS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG
Plasmid#183537PurposeVoltage indicatorDepositorInsertsomQuasAr6a-EGFP-P2A-sombC1C2TG
UseAAVTagsExpressionMutationNonePromoterhSynAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DRD2-cpHalo∆-mEGFP
Plasmid#194679PurposeCMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertDRD2_cpHalo∆
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACE2(-1119)-luc
Plasmid#31110PurposeLuciferase reporter construct for human ACE2 promoterDepositorInsertACE2 promoter (ACE2 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -1119 to +103PromoterAvailable sinceOct. 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI-neoBeta2mcherry
Plasmid#45097DepositorInsertnAChR beta2 mcherry (Chrnb2 Mouse)
UseTagshas c-myc tagExpressionMammalianMutationβ2 was cloned into pCI-neo at the restriction sit…PromoterCMVAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-ERT2-iCre-ERT2
Plasmid#178060PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.DepositorInsertERT2-iCre-ERT2
UseAAVTagsExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP
Plasmid#194686PurposeCMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFPDepositorInsertCaprola_on-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
BK alpha subunit
Plasmid#113566Purposemouse BK channel alpha subunit expression vectorDepositorInsertKcnma1 (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
Plasmid#175563PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1DepositorInsertGOXL1
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-Voltron-ST
Plasmid#119038PurposePan neuronal expression of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterelavl3Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205097Purposefusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB337
Plasmid#68061PurposeGateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoterDepositorTypeEmpty backboneUseCre/LoxTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1
Plasmid#195198Purposeall-optical silencing methodDepositorInserteHcKCR1 3.0-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1YFP28
Plasmid#41673DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 28 C-terminal-…PromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40
Plasmid#194690PurposehSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_06-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40
Plasmid#194691PurposehSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_on-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human-c-MYC-ER-mouse IRES-mcherry
Plasmid#73570PurposeMammalian retroviral expression of Myc-ERDepositorInsertcMYC (MYC Human)
UseRetroviralTagsIRES-mCherry and Mouse ERExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40
Plasmid#194692PurposehSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_off-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron-ST
Plasmid#119034PurposeExpress soma-localized Voltron in mammalian cellsDepositorInsertVoltron-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
tau exon 9–12 splicing minigene
Plasmid#121165PurposeGenerates circular RNAs from a Tau minigeneDepositorInsertTau exons 9–12 (MAPT Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FERM domain of Ezrin
Plasmid#62380PurposeExpresses the FERM domain of Ezrin (N terminal portion of the protein)DepositorInsertEzrin aa1-309 (EZR Human)
UseTagsGFPExpressionMammalianMutationcontains FERM domain of Ezrin (N terminal portion…PromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPing
Plasmid#47100PurposeExpresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.DepositorInsertsPing cDNA
mPing
hygromycin resistance gene
UsePlant expressionTagsGFPExpressionYeastMutationPromoterCaMV35s and StUbi3Available sinceSept. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)
Plasmid#174640PurposeOptogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transportDepositorInsertSSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
UseTagsSSPB(micro)-GFPExpressionMammalianMutationSSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…PromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AllCB shRNA-1
Plasmid#160065PurposeKnock Down all Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
UseTagsExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only