We narrowed to 31,648 results for: REP
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Plasmid#53108PurposeExpression of Drosophila 5-HT1BDepositorInsert5HT1B (5-HT1B Fly)
Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgRGFP
Plasmid#82695PurposeExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterPGK, U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
HES7-NLuc-2A-tdTomato
Plasmid#130932PurposeDonor vector for tagging human HES7 C-terminus with NanoLuc and tdTomatoDepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p
Plasmid#82438PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3pDepositorInserthsa-miR-27a-3p (MIR27A Human)
MutationThree copies hsa-miR-27a-3p Complementary region …Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
ExpressionBacterialAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse EMD
Plasmid#182675PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.DepositorAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT3REVIN
Plasmid#111175PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralMutationWTPromoterT3GAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-mCherry-attP(C31)-*BleoR
Plasmid#183752PurposephiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S18
Plasmid#127827PurposeProduction of recombinant human ribosomal protein S18DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria3
Plasmid#124855PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria1
Plasmid#124867PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6; mU6Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria2
Plasmid#124868PurposeMutagenesis of Gria2DepositorInsertGria2 (Gria2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin1
Plasmid#124865PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y419F
Plasmid#140317PurposeExpresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
RT3REN
Plasmid#111166PurposeTet-regulated miR-E (miR-30 variant)-based RNAi with DsRed reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralMutationWTPromoterT3GAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG
Plasmid#136513PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cellsDepositorInsertcodon-optimized AcrIIA5
TagsFLAG/NLSExpressionMammalianPromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-WT(pL84)
Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2
Plasmid#212112PurposeExpression of C-terminally tagged GABARAP(G116A) with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)
TagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of GABARAPPromoterCMVAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
TagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-TAG-HES7
Plasmid#130935PurposePiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cellsDepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W
Plasmid#105034PurposeLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
R26TV LSL-TRL
Plasmid#68476PurposeRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassetteDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseCre/Lox, Luciferase, and Mouse TargetingExpressionMammalianPromoterCAGAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70
Plasmid#62944PurposeCreating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-QF2-Hsp70
Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hORAI1sg
Plasmid#174326PurposegRNA targeting hORAI1 - encodes puromycin resistanceDepositorInserthuman Orai1 (ORAI1 Human)
UseLentiviralAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG
Plasmid#154777Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAA 150TAG in GFP reporter, hybrid PylT with …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX C_K nsp1 CoV2
Plasmid#175516PurposeExpresses C_K for bioconjugation on nsp1DepositorInsertC_K nsp1 CoV2
TagsGST tagExpressionBacterialPromotertac promoterAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-PuroR-Z
Plasmid#218904Purposegolden gate cloningDepositorInserthPGK-PuroR
ExpressionMammalianMutation246T>A and 398T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70
Plasmid#62946PurposeCreating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-QF2-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorInsertCUL1 3'UTR (CUL1 Human)
ExpressionMammalianAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS(Luc)-GFP
Plasmid#197887PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVMutationN/APromoterCAGAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-Fml1
Plasmid#193058PurposeBaculovirus expression of S.pombe Fml1 for recombinant protein productionDepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-cytoBirA-2A-mCherry_Ras
Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70
Plasmid#78306PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-nlsLexA::GADfluw-Hsp70
Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC8_GWs-Lox (VE5589)
Plasmid#163769PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.DepositorInsertno insert
Tagsno tagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
RT3REVIN
Plasmid#111167PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsDsRed and mVenusMutationWTPromoterT3GAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-S61A,N64A,T65A
Plasmid#104362PurposeExpresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
TagsmycExpressionMammalianMutationmutated the following amino acids: S61A,N64A,T65APromoterCMVAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOPO UBC
Plasmid#68456PurposeTOPO construct expressing UBC for generation of GMAP-compatible promoterDepositorAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y530EEI
Plasmid#140323PurposeExpresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studiesDepositorAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-TNNT2-Puro
Plasmid#214016PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-TNNT2-Zeo
Plasmid#214017PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 R99A CoV2
Plasmid#175515PurposeFor protein expression of R99A nsp1 CoV2DepositorInsertR99A nsp1 CoV2
TagsGST tagExpressionBacterialPromotertac promoterAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 K164A/H165A CoV2
Plasmid#175514PurposeFor protein expression of K164A/H165A nsp1 CoV2DepositorInsertK164A/H165A nsp1 CoV2
TagsGST tagExpressionBacterialPromotertac promoterAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 R124A/K125A CoV2
Plasmid#175513PurposeFor protein expression of R124A/K125A nsp1 CoV2DepositorInsertR124A/K125A nsp1 CoV2
TagsGST tagExpressionBacterialPromotertac promoterAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only