We narrowed to 5,870 results for: RIK

1. pCEP4-BG505.DS-SOSIP.4mut-gp160

   Plasmid

   #123277

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variant

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations; …

   Promoter

   CMV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-BG505.DS-SOSIP.6mut-gp160

   Plasmid

   #123278

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations; …

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Sema5a-Fc-His

   Plasmid

   #72161

   Purpose

   Expresses the extracellular region of the Sema5A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema5a (Sema5a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsDCX-EGFP-pHAT

   Plasmid

   #83918

   Purpose

   human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

   Depositor

   Gary Brouhard

   Article

   Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

   Insert

   Doublecortin isoform b (DCX Human, NM_178152.2)
   

   Use

   Tags

   6-His and EGFP+Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mGSDMD1-251

   Plasmid

   #111565

   Purpose

   Express ELANE-cleaved mouse GSDMD in mammalian cells

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

   Plasmid

   #181814

   Purpose

   Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc5b D1 (Muc5b Mouse)
   

   Use

   Tags

   TEV cleavage site followed by 6xHistidine tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXD4 (murine) MSCV

   Plasmid

   #8549

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Retroviral

   Tags

   His and T7

   Expression

   Mammalian

   Mutation

   contains T7 and HIS tags from pET; makes protein …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-HCMVgO-8his (TB40/E)

   Plasmid

   #136453

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   envelope glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HCMVgO-8his (Merlin)

   Plasmid

   #136454

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pColdI-GST-mGTSF1l

    Plasmid

    #214817

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1l

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1l (Gtsf1l Mouse)
    

    Use

    Tags

    6x His and GST

    Expression

    Bacterial

    Mutation

    Promoter

    CspA

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-CRY2–mPKCε-CAT-HA

    Plasmid

    #190483

    Purpose

    Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

    Depositor

    Diomedes Logothetis

    Article

    Gada et al J Biol Chem. 2022 May;298(5):101893. doi

    Insert

    mCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)
    

    Use

    Tags

    Fused to CRY2, HA, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTCO2-mTDGi.0

    Plasmid

    #149429

    Purpose

    miniTdg gene

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Tdg (Tdg Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    SNPs of the Tdg gene from the OLA/129 strain

    Promoter

    Tdg promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3611 pcDNA3 Bim

    Plasmid

    #8804

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

    Insert

    Bim (Bcl2l11 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

14. AdMKP-3 S197A

    Plasmid

    #131522

    Purpose

    Expresses MKP-3 with S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 197 to Alanine

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST40-CES2A-ΔC

    Plasmid

    #200554

    Purpose

    Mammalian expression of secreted mouse CES2A

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Use

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJM5-mDPYD

    Plasmid

    #61614

    Purpose

    The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidine dehydrogenase (Dpyd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Unc5c.a-Fc-His

    Plasmid

    #72180

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-RO1.7-Lrat

    Plasmid

    #206345

    Purpose

    AAV plasmid expressing LRAT in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

    Insert

    Lrat (Lrat Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.7kb human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-PTRF

    Plasmid

    #68401

    Purpose

    Expression of fluorescently tagged PTRF in mammalian cells

    Depositor

    Rob Parton

    Article

    Hill et al Cell. 2008 Jan 11;132(1):113-24. doi: 10

    Insert

    PTRF (Cavin1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA6-N-3XFLAG-Ubac2

    Plasmid

    #123597

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    ubiquitin associated domain containing 2 (Ubac2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid

    #113956

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution…

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Use

    Tags

    DHFR and myc

    Expression

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. F-MED28

    Plasmid

    #15368

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED28 (MED28 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid

    #113952

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, I132S and S212N su…

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-Susd4(3.7)-EGFP-sv40

    Plasmid

    #184413

    Purpose

    AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Susd4 gene

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

    Plasmid

    #127865

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    deleted the c-terminal 20 amino acids

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HMGA2(3'UTR DEL)+GFP

    Plasmid

    #25406

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3" UTR is absent

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-Cln6

    Plasmid

    #78109

    Purpose

    mammalian expression of mouse Cln6 with C terminal EGFP fusion

    Depositor

    Thomas Braulke

    Article

    Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

    Insert

    Cln6 (Cln6 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pASK-mSAN1-Streptag

    Plasmid

    #117165

    Purpose

    Expresses Strep-tagged murine SAN1 nuclease in bacteria

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    mSAN1 (Fam120b Mouse)
    

    Use

    Tags

    Streptag

    Expression

    Bacterial

    Mutation

    Promoter

    tetR/tetA

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mTFP1-H1-10

    Plasmid

    #55487

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-Prom1 D-5

    Plasmid

    #184017

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Sema7a-AP-His

    Plasmid

    #72049

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Mouse GFP-PRMT7 Wild Type

    Plasmid

    #65056

    Purpose

    Expresses wild type mouse GFP-PRMT7

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

40. Plxna1-AP-His

    Plasmid

    #71996

    Purpose

    Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna1 (Plxna1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. TFORF3454

    Plasmid

    #144930

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF653 (ZNF653 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAdTrack: shRev-Erbalpha

    Plasmid

    #22749

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Adenoviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

45. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

46. pAAV-DYRK2-2A-GFP

    Plasmid

    #118284

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMSCV-GSDMD_NoCys-Myc

    Plasmid

    #201169

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    C39A, C57A, C77A, C122A, C265A, C192A

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Use

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBa.GFP-KIF17 tail 400-1038

    Plasmid

    #134617

    Purpose

    Mammalian expression of mouse KIF1A 400-1038

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF17 (Kif17 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. Sema6a.a-Fc-His

    Plasmid

    #72163

    Purpose

    Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.a (Sema6a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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