161,709 results
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PPDK
Plasmid#64037PurposeExpression of Pyruvate phosphate dikinase (PPDK) in E. coliDepositorInsertpyruvate phosphate dikinase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mRhubarb713
Plasmid#197207PurposeExpresses the protein of mRhubarb713 in mammalian cellsDepositorInsertmRhubarb713
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_MCP-LSD1_Hygro
Plasmid#138457Purposelenti vector encoding the MS2-LSD1 with a 2A Hygro resistance markerDepositorInsertLSD1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mRhubarb720
Plasmid#197212PurposeExpresses the protein of mRhubarb720 in mammalian cellsDepositorInsertmRhubarb720
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag
Plasmid#228960PurposeminiEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZapLck biosensor
Plasmid#131584PurposeLck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRETDepositorInsertZapLck biosensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C
Plasmid#80932PurposeExpresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Kl-EGFP
Plasmid#45532DepositorInsertalpha Klotho (Kl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
PANK3
Plasmid#32872PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPANK3 (PANK3 Human)
UseTagsHis tag (with Thrombin cleavage)ExpressionBacterialMutationamino acids 10-370 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
SP6-VEE-IRES-Puro
Plasmid#58971Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-SCYL1-V5-miniTurbo_IDG-K
Plasmid#189911PurposeGateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertSCYL1 (SCYL1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS2118
Plasmid#49143PurposeAAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.DepositorInsertssAAV-smCBA-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromotersmCBAAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pE-35S-miniSdd7-nCas9-PBE-GmPPO2
Plasmid#204855PurposeFor cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI-mScarlet
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9Promoter35S, AtU6-26pAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAD51_Rad51
Plasmid#110031PurposeProtein expression and purification of RAD51_Rad51DepositorInsertRAD51_Rad51 (RAD51 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbGFP
Plasmid#184675PurposeNIR-Fb to GFPDepositorInsertNIR-FbGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSII316
Plasmid#35455DepositorTypeEmpty backboneUseYeast centromere vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF1216
Plasmid#144090PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNHLH1 (NHLH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-IDR
Plasmid#166451PurposeExpresses fusion of mCherry and TEAD4-IDRDepositorInsertmCherry, TEAD4-IDR(89-230) (TEAD4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-iRFPBb
Plasmid#118237PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFPDepositorInsertflagellin promoter driven iRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP1-10gly-lexADBD
Plasmid#50795PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP1-lexA DNA binding domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEA186
Plasmid#187874PurposeEmpty pVS1-based INTEGRATE vector with mCherry cargoDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vimentin-7
Plasmid#55156PurposeLocalization: Intermediate Filaments, Excitation: 587, Emission: 610DepositorInsertVimentin (VIM Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25949DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572-pBabe-Puro
Plasmid#25958DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Rab7a-7
Plasmid#58127PurposeLocalization: Ras GTPase, Excitation: 554, Emission: 581DepositorInsertRab7a (RAB7A Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-Acridone-RS1 (A7)
Plasmid#197566PurposeExpression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cellsDepositorInsertsM. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsNoneExpressionMammalianMutationN166A, V168G, W239CPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::GrRenilla
Plasmid#178319PurposeSMAD GreenRenilla luciferase reporterDepositorInsertSMAD GreenRenilla Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
UseTags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFSW FLEx mCherry-KASH
Plasmid#139652PurposeConditional expression of mCherry-KASHDepositorInsertmCherry-KASH
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro
Plasmid#25955DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25957DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-GFP-W
Plasmid#196046Purposelentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoterDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMutationPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-puro
Plasmid#45561DepositorInsertPuromycin N-acetyl transferase
UseBifunctional selection proteinTagsEGFPExpressionMammalianMutationPromoterPhcmv (Strong human cytomegalovirus immediate ear…Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1beta
Plasmid#17651DepositorInsertHP1 beta (CBX1 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFGL822
Plasmid#58225PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3343
Plasmid#144819PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRARB (RARB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-RAF1
Plasmid#23832DepositorInsertRAF1 (RAF1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
UseTagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-nMyc-wt_3'UTR
Plasmid#31858DepositorInsertnMyc wild-type 3'UTR
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterSV40Available sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
2X PRE TK luc
Plasmid#11350DepositorInsertPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-SU
Plasmid#160699PurposeExpresses GFP-BIRC3 with the short 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
