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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA neo

Plasmid

#104992

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2(1-10)

Plasmid

#82603

Purpose

Lentiviral vector for constitutive expression of sfCherry2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

SFFV

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoMyc-LATS2

Plasmid

#66852

Purpose

Express Myc-tagged LATS2 in mammalian cells

Depositor

Yutaka Hata

Article

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Insert

LATS2 (LATS2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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BPK764

Plasmid

#65767

Purpose

Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP21

Plasmid

#22574

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP21 (USP21 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pKMyc

Plasmid

#19400

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

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YFP-tagged FRB (YR)

Plasmid

#20148

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FRB (YR)

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3/UBC9

Plasmid

#20082

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

Insert

UBC9 (UBE2I Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-wt

Plasmid

#12985

Depositor

Gary Bokoch

Insert

Rac1 (RAC1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMO

Plasmid

#11970

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-M-IRES-E

Plasmid

#177938

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Promoter

Chicken B-actin

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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