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Showing: 4651 - 4700 of 11708 results

  2. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  3. cacna2d1 R241A D491A HA pcDNA3

    Plasmid
    #206109
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R241A D491A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206109-plasmid-map-sequence-id-415382

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  10. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid
    #207153
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAQ (GNAQ Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(124)E
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207153-plasmid-map-sequence-id-410285

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  18. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  19. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid
    #108375
    Purpose
    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108375-plasmid-map-sequence-id-209429

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  22. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid
    #157691
    Purpose
    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157691-plasmid-map-sequence-id-307833

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  24. pcDNA5-FRT/TO-Hygro-3xFLAG

    Plasmid
    #185359
    Purpose
    Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.
    Depositor
    Ayaz Najafov
    Insert
    3xFLAG
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185359-plasmid-map-sequence-id-363770

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  28. pcDNA3.1/Hygro-Mfn2-ActA

    Plasmid
    #191981
    Purpose
    Expression of Mfn2 tagged with mitochondrion localization signal (ActA)
    Depositor
    Luca Scorrano
    Insert
    Mfn2-ActA
    Use
    Tags
    ActA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191981-plasmid-map-sequence-id-379603

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  31. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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Showing: 4651 - 4700 of 11708 results