We narrowed to 25,909 results for: Nes
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Plasmid#87793PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only
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tet-pLKO shMKP5.v1 puro
Plasmid#87792PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9 (without sgRNA)
Plasmid#92354PurposeExpression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterCbhAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)
Plasmid#19746DepositorInsertoligo targeting p53 and PTEN (Pten Mouse)
UseCre/Lox, RNAi, and RetroviralExpressionMammalianAvailable SinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK7a1-EE
Plasmid#47578PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1DepositorInsertMKK7a1-EE (Map2k7 Mouse)
Tags3xHA and GSTExpressionBacterialMutationResidues 73-420 of RefSeq sequence. Ser271 to Gl…Available SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGabrg1
Plasmid#124870PurposeMutagenesis of Gabrg1DepositorInsertGabrg1 (Gabrg1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-CheRiff-EGFP
Plasmid#114108Purposefusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TAZ CA
Plasmid#79504PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDSDepositorAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg1
Plasmid#124856PurposeMutagenesis of Gabrg1DepositorInsertGabrg1 (Gabrg1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shId2 #2
Plasmid#83090PurposeLentiviral shRNA vector for inducible knockdown of mouse Id2DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PatoM-LOG241
Plasmid#72847PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.DepositorInsertPatoM
ExpressionBacterialPromoterPatoMAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant
Plasmid#101233PurposeBacterial expression plasmid for SpCas9 Cluster 2 variantDepositorInsertSpCas9 variant G582A/V583A/E584A/D585A/N588A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationG582A, V583A, E584A, D585A and N588APromoterT7Available SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9
Plasmid#135010PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9DepositorInserthumanized S. pyogenes Cas9
Tags3xFLAG (N terminal on insert)ExpressionMammalianPromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP2 C548A
Plasmid#126592PurposeExpresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell linesDepositorAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708/710A
Plasmid#70153Purposemammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutationsDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
Plasmid#101236PurposeBacterial expression plasmid for SpCas9 Cluster 5 + Q926A variantDepositorInsertSpCas9 variant K918A/V922A/R925A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK918A, V922A, R925A and Q926APromoterT7Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
Plasmid#79914PurposepiggyBac transposon vector, Zeocin-2A-sHRPbNLG1DepositorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRD404
Plasmid#163109PurposeExpression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmNeonGreen_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg3
Plasmid#124858PurposeMutagenesis of Gabrg3DepositorInsertGabrg3 (Gabrg3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin2a
Plasmid#124863PurposeMutagenesis of Grin2aDepositorInsertGrin2a (Grin2a Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGabrg3
Plasmid#124872PurposeMutagenesis of Gabrg3DepositorInsertGabrg3 (Gabrg3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGabrg2
Plasmid#124871PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shId2 #1
Plasmid#83088PurposeLentiviral shRNA vector for inducible knockdown of mouse Id2DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
exon L-firefly
Plasmid#81006Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
Tagsfirefly luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708A
Plasmid#70146Purposemammalian expression of MKLP1 with an eGFP fusion and an S708A mutationDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP2 WT
Plasmid#126591PurposeExpresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell linesDepositorInsertSENP2 (SENP2 Human)
TagsFLAGExpressionMammalianMutationsiRNA resistant to GAGGAGAUAUUCAGACAUUPromoterCMVAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S710A
Plasmid#70149Purposemammalian expression of MKLP1 with an eGFP fusion and an S710A mutationDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
Plasmid#101235PurposeBacterial expression plasmid for SpCas9 Cluster 4 + Q926A variantDepositorInsertSpCas9 variant F491A/M495A/T496A/N497A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationF491A, M495A, T496A, N497A and Q926APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
Plasmid#101234PurposeBacterial expression plasmid for SpCas9 Cluster 3 + Q926A variantDepositorInsertSpCas9 variant T657A/G658A/W659A/R661A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationT657A, G658A, W659A, R661A and Q926APromoterT7Available SinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
Plasmid#101220PurposeBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A, H698A and Q926APromoterT7Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
Plasmid#101232PurposeBacterial expression plasmid for SpCas9 Cluster 2 + Q926A variantDepositorInsertSpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationG528A, V583A, E584A, D585A, N588A and Q926APromoterT7Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP7.v1 puro
Plasmid#87794PurposeExpresses an inducible short hairpin targeting human MKP7 sequenceDepositorAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
ptGSVG
Plasmid#248056PurposePlant organelle-genome-specific random C-to-T mutagenesisDepositorInsertArabidopsis thaliana RPS5A promoter
ExpressionPlantMutation4 amino acid changes (S1326G, G1348S, A1398V, S14…Available SinceJune 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
mtGSVG
Plasmid#248058PurposePlant organelle-genome-specific random C-to-T mutagenesisDepositorInsertArabidopsis thaliana RPS5A promoter
ExpressionPlantMutation4 amino acid changes (S1326G, G1348S, A1398V, S14…Available SinceJune 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
mtSepCD_GSVG
Plasmid#248059PurposePlant organelle-genome-specific random C-to-T mutagenesisDepositorInsertArabidopsis thaliana RPS5A promoter
ExpressionPlantMutation4 amino acid changes (S1326G, G1348S, A1398V, S14…Available SinceJune 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pN1_RnKIF5C(1-560 d6)-mNG
Plasmid#252746PurposeExpression of a mNG-tagged, truncated (1-560) delta6 KIF5C (kinesin-1). The (1-560) is constitutively active. The delta6 mutant causes excessive damage along the microtubule lattice.DepositorAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pABDP1
Plasmid#249500PurposeBidirectional chloroplast promoter (BDP1) enabling co-expression for transformation of Chlamydomonas reinhardtii photosynthetically deficient strain CC4388DepositorInsertsmVenus (yellow/green fluorescence)
tdTomato (red fluorescence)
UseChlamydomonas reinhardtii chloroplast genomeTagsNoExpressionBacterialMutationChanged of F46L, F64L, M153T, V163A, S175G and t…PromoterBidirectional chloroplast promoter enabling co-ex…Available SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pABDP2
Plasmid#249501PurposeBidirectional chloroplast promoter (BDP2) enabling co-expression for transformation of Chlamydomonas reinhardtii photosynthetically deficient strain CC4388DepositorInsertsmVenus (yellow/green fluorescence)
tdTomato (red fluorescence)
UseSynthetic Biology; Chlamydomonas reinhardtii chlo…ExpressionBacterialMutationChange of F46L, F64L, M153T, V163A, S175G and td…PromoterBidirectional chloroplast promoter enabling co-ex…Available SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pABDP3
Plasmid#249502PurposeBidirectional chloroplast promoter (BDP3) enabling co-expression for transformation of Chlamydomonas reinhardtii photosynthetically deficient strain CC4388DepositorInsertsmVenus (yellow/green fluorescence)
tdTomato (red fluorescence)
UseChlamydomonas reinhardtii chloroplast genomeTagsNoMutationChanged of F46L, F64L, M153T, V163A, S175G and t…PromoterBidirectional chloroplast promoter enabling co-ex…Available SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTol2-cmlc2-nGFP-nCas9
Plasmid#238308PurposeExpression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.DepositorInsertCas9 (Csn1) endonuclease from Streptococcus pyogenes
UseTol2-based plasmidTagsnGFP-P2APromotercmlc2 (myl7)Available SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-QUAS:cPla2-mKate2
Plasmid#241913PurposeZebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis markerDepositorInsertQUAS enhancer driving cPla2-mKate2
UseZebrafish tol2 transgenesisTagscPla2 fused to mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only