Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,937 results for: plasmid dna

Clear keywords
Showing: 4701 - 4750 of 5937 results

  1. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

    Enlarge

  3. pHBSTC159

    Plasmid
    #124353
    Purpose
    Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    NpuDnaBΔ283 (C1G) mini-intein
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Deletion of 283-residue endonuclease domain, C1G
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124353-plasmid-map-sequence-id-242353

    Enlarge

  17. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

    Enlarge

  21. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

    Enlarge

  22. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

    Enlarge

  23. pCAT007

    Plasmid
    #171634
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171634-plasmid-map-sequence-id-336493

    Enlarge

  29. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

    Enlarge

  31. pKAR1/Pur

    Plasmid
    #23105
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene
    Depositor
    Randy Poon
    Article
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    TRE for cDNA; U6 for shRNA
    Available Since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. AAV-pgk-Cre

    Plasmid
    #24593
    Depositor
    Patrick Aebischer
    Has Service
    AAV Retrograde
    Insert
    Cre recombinase (codon optimized)
    Use
    AAV and Cre/Lox; Adeno-associated
    Promoter
    pgk
    Available Since
    May 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24593-plasmid-map-sequence-id-197583

    Enlarge

  38. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available Since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

    Enlarge

  39. GANLS

    Plasmid
    #50852
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50843-plasmid-map-sequence-id-76292

    Enlarge

Showing: 4701 - 4750 of 5937 results