19,181 results

1. pCyPet-His

   Plasmid

   #14030

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

   Insert

   CyPet
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiSAMPHv2

   Plasmid

   #167934

   Purpose

   All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psPax2-D64V-NC-mNeon

   Plasmid

   #196509

   Purpose

   mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   mNeon
   

   Use

   Tags

   mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-IP HA-Parkin

   Plasmid

   #38248

   Depositor

   Noboru Mizushima

   Article

   Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

   Plasmid

   #192453

   Purpose

   intronized ttLbCas12a for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Tags

   Expression

   Mutation

   temperature-tolerant mutation (D156R)

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKRaION2-N1

   Plasmid

   #177802

   Purpose

   Mammalian expression of green fluorescent potassium indicator KRaION2

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTP264

   Plasmid

   #149334

   Purpose

   Bacterial expression of Biotin ligase BirA

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Biotin ligase BirA
   

   Use

   Tags

   His14 and bdNEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3_eGFP

    Plasmid

    #26822

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169192

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Unspecified

    Tags

    6His-TEV

    Expression

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdEasy-1

    Plasmid

    #16400

    Purpose

    Adenoviral vector for use in AdEasy System.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mScarlet-EEA1

    Plasmid

    #169067

    Purpose

    Express human EEA1 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

    Plasmid

    #100848

    Purpose

    AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1 and AAV9

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. JD553

    Plasmid

    #160392

    Purpose

    Wheat GRF4-GIF1 chimera in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    wheat GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HaloTag-bActinCDS-bActinUTR-MS2V5

    Plasmid

    #102718

    Purpose

    Halo-Actin reporter for detection of both mRNA and protein

    Depositor

    Robert Singer

    Article

    Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    HaloTag-bActinCDS-bActin3'UTR-MS2V5
    

    Use

    Lentiviral

    Tags

    HaloTag and MS2V5

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn1-FLEX-CybSEP2-WPRE

    Plasmid

    #207661

    Purpose

    Monitoring neuropeptide release

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Cytochrome b-561 (Cyb561 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted 2 X SE…

    Promoter

    hSynapsin

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3 flag p53

    Plasmid

    #10838

    Depositor

    Thomas Roberts

    Article

    Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-mCherry-flex-dtA

    Plasmid

    #58536

    Purpose

    Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

    Depositor

    Naoshige Uchida

    Article

    Wu et al Nature. 2014 May 15;509(7500):325-30. do

    Insert

    dtA (DIP_RS18250 )
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3 HA-Sumo2 WT

    Plasmid

    #48967

    Purpose

    Expresses HA SUMO2 Wild Type in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only