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Plasmid#27512DepositorInsertOCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
469_pME_iCre
Plasmid#171792PurposepME-Vector with codon improved iCre sequence. Tol2-kit compatible.DepositorInsertcodon-optimized iCre sequence
UseTagsExpressionMutationPromoterNoneAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-dsRed
Plasmid#73460PurposeExpresses dsRed for monosynaptic circuit mappingDepositorInsertdsRed
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-Logic-AND
Plasmid#112237PurposeGFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.DepositorInsertsGFP under control of doubly-repressible promoter
GFP
araC
UseTagsExpressionBacterialMutationPromoterAND-gate and pLacIAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEx-insertion-erm
Plasmid#172210PurposeFor genetic insertion in erythromycin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseGenetic insertion in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_TtCarH-CBD_X
Plasmid#131179PurposeExpresses TtCarH-CBD (Thermus termophilus) followed by restriction sitesDepositorInsertTtCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_MxCarH-CBD_X
Plasmid#131178PurposeExpresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sitesDepositorInsertMxCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) FLIPQTV3.0 100micro
Plasmid#63730PurposeFLIPQTV3.0 glutamine sensor with D157N mutationDepositorInsertFLIPQTV3.0 sensor, 100microM affinity
UseTagsExpressionMammalianMutationD157N in glutamine binding domainPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLS.491
Plasmid#184956PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
UseTagsExpressionBacterialMutationrpoB donor T1534CPromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZY102
Plasmid#73933PurposeThis plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.DepositorInsertsbar
GUS
UseTagsExpressionPlantMutationintron is inserted into the GUS coding regionPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-empty
Plasmid#105594Purposeempty control vector with GFP marker and puro resistanceDepositorInsertno insert, empty control vector
UseRetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW533 (Peft-3::cGAL-N::let-858 3'UTR)
Plasmid#107132PurposecGAL-N driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW531 (Peft-3::cGAL-C::let-858 3'UTR)
Plasmid#107136PurposecGAL-C driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry
Plasmid#73464PurposeExpresses mCherry for monosynaptic circuit mappingDepositorInsertmCherry
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorInsertth-P2A-EGFP (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL90003
Plasmid#68262PurposeLevel 0 Golden Gate Part: Promoter U6 (Triticum aestivum)DepositorInsertPromoter: U6 (Triticum aestivum)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER50-SpCas9-ER50
Plasmid#85448PurposeExpresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cellsDepositorInsertER50-SpCas9-ER50
UseAAVTagsdestabilized domain of estrogen receptor ligand b…ExpressionMammalianMutationPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHW530 (Prab-3::cGAL-N::let-858 3'UTR)
Plasmid#107131PurposecGAL-N driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD-MyoD
Plasmid#25992DepositorInsertMyoD-MyoD homodimer (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationPromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
Plasmid#107130PurposecGAL-C driver under the control of C. elegans rab-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans rab-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-Cre
Plasmid#73474PurposeExpresses Cre for circuit mapping and genetic manipulationDepositorInsertCre recombinase
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_8-eGFP
Plasmid#107411PurposeThis plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3_8Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-G_oT7pol-IRES-BSD
Plasmid#172369PurposeGeneration of oT7pol-expressing stable cell lines, resistant to the antibiotic BlasticidinDepositorInsertoT7pol + BSD
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-FlpO
Plasmid#73473PurposeExpresses FlpO for circuit mapping and genetic manipulationDepositorInsertFlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-SADB19_oG-IRES-Puro
Plasmid#172367PurposeGeneration of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic PuromycinDepositorInsertoG + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM51559
Plasmid#153226PurposeControl plasmid for knockout of TRY and CPC genesDepositorInsertCas9, guide RNA for AP3, FAST marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA M66I
Plasmid#149687PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).DepositorInsertCapsid
UseLentiviral and RetroviralTagsExpressionMammalianMutationChanged Capsid residue Met 66 to Iso (CA M66I)PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
dCas9-SunTag(10X)-2A-BFP
Plasmid#227389PurposeAdhesion module, dCas9-SunTagDepositorInsertdCas9-SunTag
UseLentiviralTagsExpressionMutationEnzymatically dead Cas9PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAL101
Plasmid#22474PurposeExpresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF6APB
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNAPtag-LacI
Plasmid#183922PurposeLac repressor labeled with SNAPtag; can be used to label lacO sitesDepositorInsertSNAPtag-LacI (lacI Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL90002
Plasmid#68261PurposeLevel 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)DepositorInsertPromoter: U6-26 (Arabidopsis thaliana)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only