We narrowed to 5,258 results for: pcdna3.1

1. pcDNA SF-HERC2 F6

   Plasmid

   #55789

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMYC-GFP

   Plasmid

   #42142

   Depositor

   Olufunmilayo Olopade

   Article

   Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-f:PGC1

   Plasmid

   #1026

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA SF-HERC2 F4

   Plasmid

   #55787

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA SF-HERC2 F5

   Plasmid

   #55788

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. SPLICS LY-MT Long P2A

   Plasmid

   #213613

   Purpose

   Detect the long-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Klotho (secreted)

   Plasmid

   #17713

   Depositor

   Hal Dietz

   Article

   Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

   Insert

   Klotho (KL Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Hs.cargo(Cas9)

    Plasmid

    #174864

    Purpose

    Expresses human Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by HsPeg10 UTRs
    

    Use

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC3.1-caggs-Crimson-CAAX

    Plasmid

    #190041

    Purpose

    Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

    Depositor

    Michael Lin

    Article

    Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

    Insert

    Crimson
    

    Use

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mKO3-TEAD4

    Plasmid

    #221217

    Purpose

    RFP fused TEAD4

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    mKO3-TEAD4 (TEAD4 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HA Arf6 DN T27N

    Plasmid

    #10831

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 T27N (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T27N. Defective in GTP binding. Dominant negati…

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

20. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CanlonicSF

    Plasmid

    #178342

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mTq2-Calmodulin

    Plasmid

    #198200

    Purpose

    FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    Calmodulin 2 (Calm2 Rat)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3-SREBP-2

    Plasmid

    #204200

    Purpose

    Mammalian expression of SREBP-2

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-2 (SREBF2 Human)
    

    Use

    Tags

    T7x3

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GA-NES

    Plasmid

    #61018

    Purpose

    ddGFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA_RGG-GFP-RGG

    Plasmid

    #124930

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRGEN-CMV-CjCas9

    Plasmid

    #89752

    Purpose

    Expression of CjCas9 in mammalian cells

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Use

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Green Lindoblum

    Plasmid

    #163113

    Purpose

    Expresses biosensor for lactate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Lindolum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN3 (CLCN3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SPLICS PM-ER Long P2A

    Plasmid

    #164111

    Purpose

    Detect the long Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RA-NES

    Plasmid

    #61019

    Purpose

    ddRFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

39. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SPLICS PM-ER Short P2A

    Plasmid

    #164112

    Purpose

    Detect the short Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge