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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 127,146 results for: CIL

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Showing: 181 - 200 of 127146 results

  9. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    83481-plasmid-map-sequence-id-335118

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  10. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42333-plasmid-map-sequence-id-59273

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  11. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    40260-plasmid-map-sequence-id-243381

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  16. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    80899-plasmid-map-sequence-id-181185

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  18. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    144 Articles
    43860-plasmid-map-sequence-id-194368

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Showing: 181 - 200 of 127146 results