163,636 results

1. pCAG-hCas9

   Plasmid

   #51142

   Purpose

   Expresses hCas9 under the CAG promoter for CRISPR

   Depositor

   Izuho Hatada

   Article

   Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

   Insert

   hCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-CSNK1A1

   Plasmid

   #23355

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-mPD-L1-T2A-GFP

   Plasmid

   #205455

   Purpose

   lentiviral plasmid for expression of mouse PD-L1

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mPD-L1 (Cd274 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Lysosomes-20

   Plasmid

   #55073

   Purpose

   Localization: Lysosome Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167942

   Purpose

   Inducible NKX2-1 overexpression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   TTF1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

   Plasmid

   #192190

   Purpose

   HER2 CAR AAV vector PRODH2 KI (pLY080b)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
   

   Use

   AAV; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ERK2

    Plasmid

    #118296

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcdna3.1 hciap2mut

    Plasmid

    #8339

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    ciap2mut (BIRC3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    H574A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

13. GFP-N-WASP

    Plasmid

    #47406

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    N-WASP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

    Plasmid

    #60660

    Purpose

    Flp-dependent expression of mNaChBacMut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX-mNaChBacMut-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E191K

    Promoter

    EF1α

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC7A8

    Plasmid

    #132233

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A8 (SLC7A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A

    Pooled Library

    #92379

    Purpose

    CRISPRa

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Has Service

    Lentiviral Prep

    Use

    Lentiviral

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pSLQ10704

    Plasmid

    #183957

    Purpose

    U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    SKSKSO array
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ZapLck biosensor

    Plasmid

    #131584

    Purpose

    Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

    Insert

    ZapLck biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAD-iPASnFR-L

    Plasmid

    #225238

    Purpose

    Bacterial expression of iPASnFR-L

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

23. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGP-AAV-syn-jGCaMP8m-WPRE (AAV Retrograde)

    Viral Prep

    #162375-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

25. pCis mCherry-SS Himar1 A7

    Plasmid

    #84687

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX_DSB_Spectrum-V1

    Plasmid

    #192474

    Purpose

    Lentiviral DSB Spectrum V1 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRM14

    Plasmid

    #163693

    Purpose

    hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)
    

    Use

    Unspecified; Zebrafish expression

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    HSP70

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC18T-mini-Tn7T-hph-Ptac

    Plasmid

    #218364

    Purpose

    Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBpuro-ErbB3

    Plasmid

    #197362

    Purpose

    Encoding human ErbB3.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVXPuro-mGFP-Sec23B

    Plasmid

    #66598

    Purpose

    Expresses Sec23B as a fusion with mGFP

    Depositor

    David Stephens

    Article

    Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

    Insert

    Sec23B (SEC23B Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge