173,361 results
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Plasmid#63086PurposeMammalian expression of EGFP tagged FOX2DepositorAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only
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pAAV-FLEX-ArchT-tdTomato (AAV5)
Viral Prep#28305-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-FLEX-ArchT-tdTomato (#28305). In addition to the viral particles, you will also receive purified pAAV-FLEX-ArchT-tdTomato plasmid DNA. CAG-driven Cre-dependent ArchT-tdTomato expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Mito-7
Plasmid#58115PurposeLocalization: Mitochondria, Excitation: 554, Emission: 581DepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-HaloTag7-CHMP4A
Plasmid#184895PurposeStable expression of HaloTag7-CHMP4A in mammalian cellsDepositorAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVXT Sox4
Plasmid#101121PurposeSox4 coding regionDepositorInsertSOX4 (Sox4 Mouse)
ExpressionMammalianAvailable SinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV2)
Viral Prep#51502-AAV2PurposeReady-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hMTP-flag
Plasmid#138335PurposeExpresses MTPDepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-PEmax for IVT
Plasmid#178113PurposeTemplate for in vitro transcription of PEmaxDepositorInsertPEmax
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSMutationDetailed in manuscriptPromoterT7 (inactivated)Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KL-mcherry
Plasmid#116947PurposeIntegrating mCherry into lambda attB in E. coli chromosome.DepositorInsertmcherry
Available SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF15_P2A_Hygro_Barcode
Plasmid#120501PurposeBarcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDR119
Plasmid#13332DepositorAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis
Plasmid#156912PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertIGSF8 (IGSF8 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Hygro
Plasmid#85972PurposeTet/Dox inducible shRNA lentivirus with hygromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiPromoterH1 / TetOAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt7B
Plasmid#35915DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralExpressionMammalianPromoterEF1-a and U6Available SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSNK2A1
Plasmid#23678DepositorInsertCSNK2A1 (CSNK2A1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pWZL hygro H-Ras V12
Plasmid#18749DepositorAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA
Plasmid#158988PurposeVector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neuronsDepositorInsertdSaCas9-KRAB
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available SinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgM/κ
Plasmid#61881PurposeExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)DepositorInsertsHER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Neurofascin-186-HA
Plasmid#31061DepositorAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.BB.28.3z
Plasmid#200681PurposeModular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-C563A-C596A
Plasmid#171220PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.DepositorInsertAR-C563A-C596A (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C563A-C596APromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-C619Y
Plasmid#171222PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.DepositorInsertAR-C619Y (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-C619YPromoterEF-1aAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-PheRSa-PheRSb
Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)
Plasmid#30123DepositorTypeEmpty backboneTagsHis6-MBP-N10-TEVExpressionInsectAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLXRN-EGFP
Plasmid#99207PurposeEGFP expressing bicistronic retroviral vectorDepositorInsertEGFP
UseRetroviralExpressionMammalianMutationA Kozak sequence was incorporated to enhance tran…PromoterLTRAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-SNAPf (w878-1)
Plasmid#29652DepositorTypeEmpty backboneUseEntry vectorTagsSNAPfAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_pGK_LaG17_synNotch_Gal4VP64
Plasmid#79127PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertLaG17_synNotch_Gal4DBDVP64
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRExpressionYeastPromoter5S rRNA-tRNA(Arg)Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
DH10B-M.Osp807II
Bacterial Strain#114267PurposeThe strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.DepositorBacterial ResistanceBleocin (Zeocin)Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
10TALE_Pmin_fLuc
Plasmid#160663PurposeExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domainDepositorInsert10TALE_Pmin_fLuc
UseLuciferaseExpressionMammalianPromoterminimalAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_deltaUBA_UBAP2L_mCherry
Plasmid#105287Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2LDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianMutationdeleted amino acids 20-108PromoterTRE promoter, Tet ONAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only