We narrowed to 13,451 results for: Mpl;

1. pEBTetD-β-tubulin_SNAP

   Plasmid

   #136832

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   TUBB_SNAP (TUBB Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

   Plasmid

   #127360

   Purpose

   gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   R118G

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI-neo-Myc-hAtg14

   Plasmid

   #24293

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   autophagy related genes 14 (ATG14 Human)
   

   Tags

   3xMyc

   Expression

   Mammalian

   Available Since

   Sept. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pGAD-Atg17

   Plasmid

   #121386

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Expression

   Yeast

   Promoter

   ADH1 promoter

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MEIS1B-MSCV

   Plasmid

   #21022

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 1999 Apr . 19(4):3051-61.

   Insert

   MEIS1B (Meis1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pHis-hPim1

   Plasmid

   #100722

   Purpose

   Expression of human Pim1 kinase domain in E.coli

   Depositor

   Byung Il Lee

   Article

   Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

   Insert

   Pim1 (PIM1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 29-313 of isoform 2

   Promoter

   T7 promotor

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HS-DN-Ect2

   Plasmid

   #105956

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   Ect2 (ECT2 Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Promoter

   CMV and mouse U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

   Plasmid

   #199361

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

    Plasmid

    #199362

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOstar-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNIC-Bio2_SMAC(delta(55))_9His

    Plasmid

    #197976

    Purpose

    E. coli expression of SMAC(delta(55))-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55)

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-FLAG-BMAL1-S516A

    Plasmid

    #186833

    Purpose

    Expresses FLAG-BMAL1-S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 516 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-FLAG-BMAL1-S519A

    Plasmid

    #186834

    Purpose

    Expresses FLAG-BMAL1-S519A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 519 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVH016

    Plasmid

    #80400

    Purpose

    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFPC1/GgVcl 1-851 A50I mutant

    Plasmid

    #46269

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I mutation (VCL Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    1-851 (Vh) head domain, A50I mutation

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmcsg7-mCRT(WT)

    Plasmid

    #83505

    Purpose

    Bacterial expression of Calreticulin

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. K8.1Pr-pGL4.16

    Plasmid

    #131037

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV K8.1 100 bp promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pfastbac/mBmal62-447

    Plasmid

    #47335

    Purpose

    mBmal fragment cloned into pfastbac and crystallized

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Mutation

    aa 62-447

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pBW300ParsR-Amp32T

    Plasmid

    #78652

    Purpose

    pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
    

    Use

    Synthetic Biology

    Promoter

    see insert

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pEB1deltaC-2xEGFP shRNA resistant

    Plasmid

    #37925

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion after aa248, TTCCTGT=>TCCCGGT

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pscALPS-SIV-RCM vpx

    Plasmid

    #115812

    Purpose

    Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM Vpx (AF349680)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEBTet-SNAP-FOP

    Plasmid

    #136796

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-FGFR
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBacPAK8-ElonginB

    Plasmid

    #29504

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Elongin B (ELOB Human)
    

    Expression

    Insect

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pET21-MRPP1_native+

    Plasmid

    #67865

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form)

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP1 (TRMT10C Human)
    

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGoldyTALEN-TUBA1B-TAL-2

    Plasmid

    #62854

    Purpose

    TALEN mediated gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGoldyTALEN-TUBA1B-TAL-1

    Plasmid

    #62853

    Purpose

    TALEN mediated gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159855

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p6896 PHAGE-P CMVT N-HA-hE6AP II wt

    Plasmid

    #37603

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP II wt (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF-DEST51 HA-PNRC1

    Plasmid

    #123294

    Purpose

    Mammalian expression of PNRC1 N-HA

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pscALPS-SIV-AGM-VER9063 vpr

    Plasmid

    #115825

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (Ver 9063)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-C1X-Nup93-mut

    Plasmid

    #87335

    Purpose

    To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmAGO1

    Plasmid

    #22420

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

    Insert

    Argonaut-1, variant A (AGO1 Fly)
    

    Use

    Drosophila expression

    Tags

    lambdaN-HA

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLBM0090

    Plasmid

    #172824

    Purpose

    Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    [35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
    

    Use

    Synthetic Biology

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3 Flag Rab1a N124I

    Plasmid

    #46778

    Purpose

    expression of N124I Rab1a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab1a (Rab1a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N124I cannot stably bind guanine nucleotide

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

42. Flag-ATXN1_V591A_S602D

    Plasmid

    #48190

    Purpose

    Expresses V591A and S602D mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Flag-ATXN1_V591A_L686E

    Plasmid

    #48191

    Purpose

    Expresses V591A and L686E mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Flag-ATXN1_S602D_L686E

    Plasmid

    #48192

    Purpose

    Expresses S602D and L686E mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJZC625

    Plasmid

    #62321

    Purpose

    sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA +1x MS2, Pol II promoter with ribozyme cleavage
    

    Expression

    Yeast

    Promoter

    pAdh

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEBTetD-SNAP-FOP

    Plasmid

    #136797

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-FGFR
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. Sha2Cas9-HF

    Plasmid

    #192142

    Purpose

    Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Sha2Cas9-HF (R247A and N415A)

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. Sha2Cas9

    Plasmid

    #192140

    Purpose

    Expresses Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. SpeCas9

    Plasmid

    #192139

    Purpose

    Expresses SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. A3H HapII-Cas9n-UGI

    Plasmid

    #119141

    Purpose

    Base editor made from APOBEC3H Haplotype II

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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