We narrowed to 6,990 results for: Cad

1. GAL FLAG25QDProGFP p306

   Plasmid

   #15831

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

2. GFP-CBS (WT)

   Plasmid

   #182923

   Purpose

   Mammalian expression plasmid for human CBS

   Depositor

   Philip Eaton

   Article

   Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

   Insert

   Cystathionine Beta-Synthase (CBS Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSALECTNK-TEM1(S70A,D179G)/csTEM1

   Plasmid

   #81163

   Purpose

   pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   Codon swapped TEM-1 from H26 to W290 and His6x

   Expression

   Bacterial

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   lac promoter

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GAL FLAG72QDProGFP p306

   Plasmid

   #15833

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 72Q Delta Pro (HTT Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pQE-80L MBP-SspB Micro

   Plasmid

   #60410

   Purpose

   E. coli expression of SspB Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB micro
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   R73Q, Y11K and A15E

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAK184

   Plasmid

   #167281

   Purpose

   MazF-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Pxyl

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSara-C

   Plasmid

   #60722

   Purpose

   Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 180-880 of T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Only amino acids 180-880 of T7 RNAP

   Promoter

   PBAD

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSara-N

   Plasmid

   #60721

   Purpose

   Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 1-179 of T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Only amino acids 1-179 of T7 RNAP

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Neo-Bam E2F6

   Plasmid

   #37965

   Depositor

   Jacqueline Lees

   Article

   Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

   Insert

   E2F6 (E2F6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. E3020X

    Plasmid

    #21366

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed E3020 to stop codon

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

11. sIL6R PLAK1

    Plasmid

    #161519

    Purpose

    Expresses soluble IL6 receptor in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    soluble IL-6 receptor
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-mScarlet-SARS-CoV-2-5'UTR

    Plasmid

    #184638

    Purpose

    Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pENTR223-BORF2

    Plasmid

    #195834

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BORF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BORF2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SETD2(SET)-Cas9

    Plasmid

    #186700

    Purpose

    Plasmid encoding SETD2-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETD2(SET)-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4-Beclin1 1-150

    Plasmid

    #24389

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-150.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

16. 1127-DP NTP wild type

    Plasmid

    #32398

    Depositor

    Kathleen Green

    Article

    Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains only the first 584 aa

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pcFLAG RUNX3 (R122C)

    Plasmid

    #203438

    Purpose

    Mammalian expression of RUNX3

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-ELL pBacPAK8

    Plasmid

    #15322

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pQE-80L iLID (C530M)

    Plasmid

    #60408

    Purpose

    E. coli expression of iLID (C530M)

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    iLID C530M
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C530M

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

21. MSCVhygro-F-Ezh2-F667I

    Plasmid

    #24927

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Ezh2 (EZH2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phenylanaline(F)667 mutated to Isoleucine(I)

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1(-)-EGFP-FP4-mito

    Plasmid

    #229692

    Purpose

    Transient expression of EGFP FP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Mitochondira FP4 motifs from Listeria ActA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(-)-EGFP-AP4-mito

    Plasmid

    #229693

    Purpose

    Transient expression of EGFP AP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (mitochondria)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-Map7-fulllength

    Plasmid

    #46076

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-fulllength (Map7 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. [UBC][firefly luciferase][target cloning][renilla luciferase]

    Plasmid

    #97190

    Purpose

    Reporter for translation activation via Pum.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    Firefly Luciferase
    Renilla Luciferase
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-INPP4A

    Plasmid

    #171944

    Purpose

    Expresses INPP4A in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP4A (INPP4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-CRY2-OCRL

    Plasmid

    #79565

    Purpose

    expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

    Depositor

    Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    OCRL (OCRL Human)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    aa 234_539

    Promoter

    CMV

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 PARL-FLAG-CT S277G

    Plasmid

    #13615

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic Serine 277 to Alanine

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pROSA26-promoter

    Plasmid

    #21710

    Depositor

    Philippe Soriano

    Article

    Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

    Insert

    ROSA26 promoter (Gt(ROSA)26Sor Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-tLuc-mCherry

    Plasmid

    #107553

    Purpose

    tLuc-mCherry in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge