163,977 results

1. pX330GFP-hU6-gRNA-hSpCas9

   Plasmid

   #128385

   Purpose

   GFP positive gRNA/hSpCas9 construct

   Depositor

   Budd A. Tucker

   Article

   Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

   Insert

   GFP, gRNA and hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKJ0463

   Plasmid

   #228715

   Purpose

   Bacterial expression of epT7 RNAP; for protein production and purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Science 0, eadr6006

   Insert

   Sumo_epT7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T3M/G47A/E643G

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Luciferase-pcw107-V5

   Plasmid

   #64649

   Purpose

   (control) when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   n/a
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   PGK

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223_EGFR_p.ELREA_746del

   Plasmid

   #82911

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Deletion of residues 746-750 (ELREA)

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sigma 70 promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

   Plasmid

   #171437

   Purpose

   Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   c-Fos-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Use

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

   Plasmid

   #169187

   Purpose

   To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mito-CanlonicSF/pCMV-myc-mito

    Plasmid

    #224465

    Purpose

    To explore mitochondrial lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Rauseo et al bioRxiv 2024.07.19.604361

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHAGE-TO-nmdCas9-3XGFP

    Plasmid

    #64109

    Purpose

    Expression of Nm dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.2-FUS-1-526aa-V5

    Plasmid

    #29609

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIN4-EF2-O2S

    Plasmid

    #21162

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4, Sox2 (SOX2, POU5F1 Human)
    

    Use

    Lentiviral; Reprogramming

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. SmoM2 (W535L)-pcw107-V5

    Plasmid

    #64628

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    SMO (SMO Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    W535L

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171594

    Purpose

    Strong human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217345

    Purpose

    crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
    crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
    crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
    crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1218

    Plasmid

    #142157

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRRX1 (PRRX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBGSA C5aR1-EGFP

    Plasmid

    #62612

    Purpose

    Constitutive expression of C5aR1-EGFP in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Suvorova et al Traffic. 2005 Feb;6(2):100-15.

    Insert

    C5aR1 (C5AR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pAAV hPGRN

    Plasmid

    #213682

    Purpose

    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    Human Progranulin (hPGRN) (GRN Human)
    

    Use

    AAV

    Tags

    Twin-Strep tag and V5 tag (after signal peptide)

    Expression

    Mutation

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUCmini-iCAP-AAV.CAP-B22

    Plasmid

    #175005

    Purpose

    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1_Lifeact-EGFP

    Plasmid

    #67303

    Purpose

    Fluorescent indicator for F-actin

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Lifeact-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLIK Tgfbr3-HA neo

    Plasmid

    #58508

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

25. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAD-pHScarlet

    Plasmid

    #191258

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

    Depositor

    Robert Campbell

    Insert

    pHScarlet
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid

    #99231

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CaMKII

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GST-Pin1

    Plasmid

    #19027

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

    Insert

    Pin1 (PIN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pREDusk-AmpR-MCS

    Plasmid

    #188974

    Purpose

    For red light-repressed bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HLA_DRB1_07_01

    Plasmid

    #165014

    Purpose

    Expression of HLA-DRB1*07:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRB1*07:01 (HLA-DRB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge