166,365 results

1. pH2B-emiRFP670

   Plasmid

   #136571

   Purpose

   Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   H2B-emiRFP670
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Use

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mARGAna (cassette 2)

   Plasmid

   #191342

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307-TMPRSS2-G418

   Plasmid

   #158459

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPbcas13b

   Plasmid

   #89906

   Purpose

   Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-KDM2B-P2A-mCherry

    Plasmid

    #203852

    Purpose

    Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    KDM2B (KDM2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mouse sgRNA library Brie in lentiCRISPRv2

    Pooled Library

    #73632

    Purpose

    Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. TFORF2957

    Plasmid

    #144433

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2003

    Plasmid

    #142902

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXD3 (FOXD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYL156 (TRV RNA2)

    Plasmid

    #148969

    Purpose

    Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    Modified TRV RNA2
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    see comments section below

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-pBAD

    Plasmid

    #54762

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. peGFP-N1-Cofilin

    Plasmid

    #186747

    Purpose

    Expresses wildtype GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMD2.G-VSV-G-mut

    Plasmid

    #196508

    Purpose

    VSV-G mutant (K47Q, R354A)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    VSV-G
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-D50 lamin A

    Plasmid

    #17653

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

    Insert

    D50 lamin A (LMNA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50 (50 aa internal deletion in globular tai…

    Promoter

    CMV

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pE-SUMO CRYAA

    Plasmid

    #80756

    Purpose

    Expression of human alpha A-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYAA (CRYAA Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JpExpress404 PTEN-LONG

    Plasmid

    #49417

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN-Long (PTEN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PE-SUMO CRYBB1

    Plasmid

    #217675

    Purpose

    For expression of SUMO tagged human beta B1 crystallin

    Depositor

    Larry David

    Insert

    CRYBB1 (CRYBB1 Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNTI935

    Plasmid

    #216638

    Purpose

    cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-CMV-GFP

    Plasmid

    #67634

    Purpose

    AAV vector expressing GFP

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-flex-taCasp3-TEVp

    Plasmid

    #45580

    Depositor

    Jim Wells, Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Has Service

    AAV1 and AAV5

    Inserts

    Caspase 3 (CASP3 Human, Synthetic)
    Tobacco Etch Virus protease
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Linker replaced with a TEV protease cleavage site

    Promoter

    EF1a

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti.PGK.LifeAct-GFP.W

    Plasmid

    #51010

    Purpose

    Lentivector that expresses LifeAct-GFP from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-GFP
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-VSVN

    Plasmid

    #64087

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV nucleoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid

    #110556

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    anit-RD114 light chain
    

    Use

    Tags

    mKappa

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge