We narrowed to 23,817 results for: Spr

1. pAN-AND

   Plasmid

   #62308

   Purpose

   synthetic circuit: AND gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Inserts

   A1NT sgRNA
   A4NT sgRNA
   A2NT sgRNA
   A1NT
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PA2, PPhlF, pA4, and pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_FokI-1x7

   Plasmid

   #63607

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLsCas13a-gRNA-1

   Plasmid

   #164865

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_(GLuc)_sgRNA

   Plasmid

   #68422

   Purpose

   Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Gluc sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p426-SNR52p-gRNA.clr-2.Y-SUP4t

   Plasmid

   #89692

   Purpose

   gRNA for clr-2 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for clr-2 locus
   

   Use

   CRISPR; Grna

   Tags

   No

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458_NR2F6_2

   Plasmid

   #86343

   Purpose

   Encodes gRNA for 3' target of human NR2F6

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against NR2F6 (NR2F6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458_NR2F6_1

   Plasmid

   #86342

   Purpose

   Encodes gRNA for 3' target of human NR2F6

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against NR2F6 (NR2F6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ158

   Plasmid

   #69307

   Purpose

   Gateway entry vector with hSpCas9 (Human codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9 (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA EMX1

    Plasmid

    #128121

    Purpose

    Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA EMX1 + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC548

    Plasmid

    #62316

    Purpose

    sgRNA with 1x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x PP7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330A_FokI-1x6

    Plasmid

    #63606

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEL089

    Plasmid

    #137897

    Purpose

    iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_ZNF331_1

    Plasmid

    #135739

    Purpose

    Encodes gRNA for 3' target of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 gRNA (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_ZNF331_2

    Plasmid

    #135740

    Purpose

    Encodes gRNA for 3' target of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 gRNA (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB1C3-hsg(M)

    Plasmid

    #138262

    Purpose

    Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    sgRNA M
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_SAP130_1

    Plasmid

    #86310

    Purpose

    Encodes gRNA for 3' target of human SAP130

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against SAP130 (SAP130 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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