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Plasmid#211562PurposeAmber Suppressor tRNA for HistidineDepositorInserttRNAHis
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLeu57
Plasmid#211563PurposeAmber Suppressor tRNA for LeucineDepositorInserttRNALeu
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLys57-1
Plasmid#211564PurposeAmber Suppressor tRNA for LysineDepositorInserttRNALys
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLys57-2
Plasmid#211565PurposeAmber Suppressor tRNA for Lysine (Methionine-derived)DepositorInserttRNALys
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLys57-3
Plasmid#211566PurposeAmber Suppressor tRNA for Lysine (Isoleucine-derived)DepositorInserttRNALys
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLys57-4
Plasmid#211567PurposeAmber Suppressor tRNA for Lysine (Aspartate-derived)DepositorInserttRNALys
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPhe57
Plasmid#211568PurposeAmber Suppressor tRNA for PhenylalanineDepositorInserttRNAPhe
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPro57
Plasmid#211569PurposeAmber Suppressor tRNA for ProlineDepositorInserttRNAPro
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSer57
Plasmid#211570PurposeAmber Suppressor tRNA for SerineDepositorInserttRNASer
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAla57
Plasmid#211556PurposeAmber Suppressor tRNA for AlanineDepositorInserttRNAAla
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pArg57
Plasmid#211557PurposeAmber Suppressor tRNA for ArginineDepositorInserttRNAArg
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCys57
Plasmid#211558PurposeAmber Suppressor tRNA for CysteineDepositorInserttRNACys
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGln57
Plasmid#211559PurposeAmber Suppressor tRNA for GlutamineDepositorInserttRNAGln
UseTagsExpressionBacterialMutationPromoterSynthetic (Miller 1986)Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABCB10-ChR2-eYFP
Plasmid#206037PurposeExpress ChR2 in mitochondriaDepositorInsertChR2
UseTagsABCB10 mitochondrial leading sequence and EYFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-zFGFR1a
Plasmid#232639PurposeZebrafish FGFR1a receptor kinase domain fused to VfLOVDepositorInsertzFGFR1a kinase domain + VfLOV domain
UseTagsExpressionMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
EK0479 CMV-TO-VEGF-A(165) (FLP-IN)
Plasmid#233384PurposeSecreted human VEGF-ADepositorInsertVEGF-A (VEGFA Human)
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-iCre
Plasmid#232773PurposeIVT template for making modRNA encoding iCreDepositorInsertiCre
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-His-VSIR
Plasmid#232663Purposetransient expresses VSIR in mammalian cellsDepositorInsertVSIR (C10orf54 Human)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Hyg
Plasmid#232091PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRTagsExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_Divergent Donor
Plasmid#229784PurposeBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_Divergent Donor
Plasmid#229786PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG-mRuby2
Plasmid#229801PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
PDG458 V3
Plasmid#226959PurposeCBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-rM1PYK
Plasmid#220232PurposeInducible expression of rabbit muscle pyruvate kinase isoform 1DepositorInsertRabbit muscle pyruvate kinase (PKM )
UseTags6xHis-MBP-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-hLPYK
Plasmid#220231PurposeInducible expression of human liver pyruvate kinaseDepositorInsertHuman liver pyruvate kinase (PKLR Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorUseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-804_HA-GD2-28z_CAR_BATF-TFAP4
Plasmid#207491PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertBATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3XHA-p53 Δ1-42
Plasmid#196269PurposeMammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)DepositorInsertp53 Δ1-42 (Trp53 Mouse)
UseTags3xHAExpressionMammalianMutationdeleted amino acids 1 to 42PromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-HLA-B-Fc(DAPA)-AviTag-6xHis
Plasmid#156835PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertHLA-B (HLA-B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-Electra2
Plasmid#184928PurposeKeratin labeling with blue fluorescent protein Electra2DepositorInsertKeratin-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT2-Linker-eGFP
Plasmid#176085PurposeEGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassetteDepositorInsertBRCT2 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT1-Linker-eGFP
Plasmid#176074PurposeEGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassetteDepositorInsertBRCT1 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-FHA-Linker-eGFP
Plasmid#176065PurposeEGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassetteDepositorInsertFHA
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP-GFP
Plasmid#73579PurposeLentivirus expression of mOC-STAMPDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only