We narrowed to 13,592 results for: crispr cas9

1. pX459V2.0-SpCas9-HF4

   Plasmid

   #108295

   Purpose

   pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdSh.PGK.Cas9

   Plasmid

   #58253

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   Human codon-optimized S. pyogenes Cas9
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. OmEF1aCas9P2ApuroSB

   Plasmid

   #165484

   Purpose

   Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

   Depositor

   Dietmar Kueltz

   Article

   Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

   Insert

   Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mutation

   Promoter

   Oreochromis mossambicus EF1 alpha promoter (OmEF1…

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9GG

   Plasmid

   #131009

   Purpose

   Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   promoter PJ23119
   mRFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-sgRNA-Cas9

   Plasmid

   #49330

   Purpose

   Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH47742:FCP:Cas9YFP

   Plasmid

   #85986

   Purpose

   Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR; Golden gate assembly

   Tags

   SV40 NLS, YFP

   Expression

   Mutation

   Promoter

   FCP

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ8-P_dCas9

    Plasmid

    #74063

    Purpose

    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Propionate inducible promoter (prp)

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_dCas9-2xAM_hIRF-1

    Plasmid

    #92221

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9FnCpf1GG

    Plasmid

    #131013

    Purpose

    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSU-araC-Cas9

    Plasmid

    #120423

    Purpose

    Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid

    #101230

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid

    #101231

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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