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We narrowed to 7,330 results for: Ank

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Showing: 6701 - 6750 of 7330 results

  3. pNIC28-Bsa4_ZV-NS5-RdRp

    Plasmid
    #179847
    Purpose
    The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89
    Depositor
    Julien Lescar
    Article
    Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00
    Insert
    Zika Virus Non-structural protein 5 RdRp domain
    Tags
    6-histine
    Expression
    Bacterial
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179847-plasmid-map-sequence-id-365329

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  4. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  7. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid
    #207759
    Purpose
    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207759-plasmid-map-sequence-id-415319

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  9. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124537
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124537-plasmid-map-sequence-id-242309

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  12. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid
    #191334
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191334-plasmid-map-sequence-id-381755

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  13. pPBC-LG3-tdT

    Plasmid
    #62810
    Purpose
    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    Expression
    Bacterial and Mammalian
    Promoter
    CAG
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62810-plasmid-map-sequence-id-123118

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  15. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid
    #227306
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227306-plasmid-map-sequence-id-452076

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  19. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124542
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124542-plasmid-map-sequence-id-242329

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  20. pMDC32B-AtTAS1c-D2-B/c

    Plasmid
    #137884
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    2x35S
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137884-plasmid-map-sequence-id-275811

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  21. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  22. pET24a-VHH-tev-std

    Plasmid
    #109423
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109423-plasmid-map-sequence-id-260701

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  24. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124543
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124543-plasmid-map-sequence-id-242336

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  25. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  27. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

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  28. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid
    #207786
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207786-plasmid-map-sequence-id-413796

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  29. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid
    #79885
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79885-plasmid-map-sequence-id-150051

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  30. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  31. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Expression
    Bacterial
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

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  32. pBetaActin-KIF13aMotor-mScarlet-FKBP

    Plasmid
    #191337
    Purpose
    Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    KIF13aMotor-mScarlet-FKBP (KIF13A Human)
    Expression
    Mammalian
    Mutation
    motor domain aa 1-639
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191337-plasmid-map-sequence-id-381769

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  33. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  34. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  35. pcDNA-Prom1 D-5

    Plasmid
    #184017
    Purpose
    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.
    Depositor
    Peter Stoilov
    Article
    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d
    Insert
    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Deletion of exon 19a (amino acids 696-701), and d…
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184017-plasmid-map-sequence-id-362468

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  38. p221- purVSG117UTR

    Plasmid
    #59732
    Purpose
    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.
    Depositor
    Gloria Rudenko
    Article
    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:
    Insert
    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    Use
    For homologous recombination into vsg221 expressi…
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59732-plasmid-map-sequence-id-100249

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  39. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137883-plasmid-map-sequence-id-275808

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  41. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid
    #124541
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    Expression
    Mammalian
    Mutation
    WT, WT
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124541-plasmid-map-sequence-id-242326

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  42. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124539
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55A, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124539-plasmid-map-sequence-id-242319

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  45. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

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  47. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124544
    Purpose
    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55A, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124544-plasmid-map-sequence-id-242339

    Enlarge

  48. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124540
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124540-plasmid-map-sequence-id-242322

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  49. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid
    #124538
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41
    Promoter
    Minimal ADE
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124538-plasmid-map-sequence-id-242317

    Enlarge

  50. pFLAG-WTS-1

    Plasmid
    #66856
    Purpose
    Express FLAG-tagged C.elegans WTS-1 in mammalian cells
    Depositor
    Yutaka Hata
    Article
    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.
    Insert
    WTS-1 (wts-1 Nematode)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E153G and D871G mutations in WTS1 compared to Gen…
    Promoter
    CMV
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6701 - 6750 of 7330 results