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Showing: 6851 - 6900 of 11732 results

  2. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

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  3. pDUAL_2_araBAD

    Plasmid
    #155176
    Purpose
    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155176-plasmid-map-sequence-id-310563

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  4. pBICI_2_strong

    Plasmid
    #155168
    Purpose
    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155168-plasmid-map-sequence-id-310553

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  9. pAL942

    Plasmid
    #119721
    Purpose
    precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
    Tags
    none
    Expression
    Bacterial
    Mutation
    Alanine 248 to Threonine, Alanine 188 to Cysteine…
    Promoter
    T7
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119721-plasmid-map-sequence-id-232465

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  11. pUC19-B8I085

    Plasmid
    #103089
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103089-plasmid-map-sequence-id-201739

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  14. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  23. C20-His-bio

    Plasmid
    #52327
    Purpose
    Expresses full-length Adipocyte plasma membrane-associated protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    C20 (APMAP Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52327-plasmid-map-sequence-id-83058

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  24. TXNDC-bio-His

    Plasmid
    #51858
    Purpose
    Expresses full-length Thioredoxin-related transmembrane protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TXNDC (TMX1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51858-plasmid-map-sequence-id-79746

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  25. pChlr-2SR

    Plasmid
    #52146
    Purpose
    Encodes module 2 with amino acids 12S-16R for recognition of nt 7C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pChlr-3NQ

    Plasmid
    #52149
    Purpose
    Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 3
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pChlr-4CQ

    Plasmid
    #52151
    Purpose
    Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pChlr-4NYQ

    Plasmid
    #52157
    Purpose
    Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 13H to 13Y in Module 4
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pChlr-5NQ

    Plasmid
    #52161
    Purpose
    Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pChlr-6CQ

    Plasmid
    #52163
    Purpose
    Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Promoter
    human Synapsin 1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  40. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

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  43. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

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  44. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Tags
    2xSTII
    Expression
    Insect
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  45. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid
    #203802
    Purpose
    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T23N
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203802-plasmid-map-sequence-id-401645

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  48. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  50. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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Showing: 6851 - 6900 of 11732 results