164,447 results
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Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV8)
Viral Prep#50465-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GRP78/BiP
Plasmid#32701DepositorInsertGRP78/BiP (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP1
Plasmid#188896PurposeExpress 6xHis-Tag miniGFP1 in E.coliDepositorInsertminiGFP1
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via ā¦ExpressionMammalianMutationD10A, H840APromoterCMV and U6Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorInsertSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C3)-Nup153
Plasmid#64268PurposeGFP-tagged mammalian expression of Nup153DepositorInsertNuP153 (NUP153 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-Lyn11-mEGFP-cpHaloā-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett
Plasmid#205703PurposeCMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHaloā and Hpep1-FRB-mScarlett) in mammalian cell linesDepositorInsertLyn11-EGFP-FKBP-cpHaloā-P2A-Hpep1-FRB-mScarlett
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-GRAM-W
Plasmid#211704PurposeLentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)DepositorInsertEGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJC005.em
Plasmid#182738PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)
Viral Prep#131004-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCR5
Plasmid#98943PurposeMammalian expression plasmid for untagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription oā¦TagsFlanked by 5' and 3' UTRs from Xenopus ā¦ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
UseTagsHis-TagExpressionBacterialMutationPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.eGFP.WPRE.hGH (AAV5)
Viral Prep#105549-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.GFAP.eGFP.WPRE.hGH (#105549). In addition to the viral particles, you will also receive purified pAAV.GFAP.eGFP.WPRE.hGH plasmid DNA. GFAP-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsEGFPAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (AAV1)
Viral Prep#101060-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (#101060). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGGā¦PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-Calreticulin-N-16
Plasmid#55892PurposeLocalization: Endoplasmic Reticulum, Excitation: 559, Emission: 600DepositorInsertCalreticulin (CALR Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-ERBB3
Plasmid#116735PurposeLentiviral expression of ERBB3DepositorInsertERBB3 (ERBB3 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorUseTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
4XCLEAR-luciferase reporter
Plasmid#66800PurposeReporter for activity of the CLEAR networkDepositorInsert4XCLEAR
UseLuciferaseTagsExpressionMutationPromoterFour CLEAR elements (GTCACGTGAC) in tandem deriveā¦Available sinceNov. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic NAD+ biosensor
Plasmid#186787PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasmDepositorInsertcytoplasmic NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV1)
Viral Prep#100833-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits