We narrowed to 10,954 results for: aga

1. iRFP713-N2-Frankenbody-Flag

   Plasmid

   #175291

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with iRFP713
   

   Use

   Tags

   iRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. STARR-seq luciferase validation vector_SCP1_empty

   Plasmid

   #99299

   Purpose

   Luciferase validation vector; empty vector without insert to determine baseline

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMx/ATP11C-HA

   Plasmid

   #209237

   Purpose

   Mammalian expression of ATP11C

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. KTK_354

   Plasmid

   #180525

   Purpose

   KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMP-Bmi1_3

   Plasmid

   #36354

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pTXB1-H2B(1-114)_wt

   Plasmid

   #118252

   Purpose

   Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   xlH2B(1-114)
   

   Use

   Tags

   Mxe-GyrA intein fused to chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-HA-BLMP-1-pcDNA3.1-

   Plasmid

   #52512

   Purpose

   expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Blmp-1 (blmp-1 Nematode)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   N75S mutation compared GenBank reference sequence…

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCX-SponGee-Kras

    Plasmid

    #134777

    Purpose

    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee-Kras
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAT15415-BEAR-GFP-target-mCherry

    Plasmid

    #162995

    Purpose

    plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    sgRNA targeting the BEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Use

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PBLO CasX-gRNA

    Plasmid

    #126419

    Purpose

    E. coli vector for CasX gRNA --- Used for In Vitro Transcription

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    casX gRNA
    

    Use

    CRISPR; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPGP_scFv

    Plasmid

    #183732

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Use

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    Promoter

    GAP promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmyc-GFP-TNRC6A-NES-mut

    Plasmid

    #42001

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-PIG-N3FLAG-TAF10

    Plasmid

    #105990

    Purpose

    retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF10 (Taf10 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLK1 G5.1 gRNA

    Plasmid

    #90834

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK1 (Guide Designation G5.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-SP110C

    Plasmid

    #65393

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110C
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3HA-ErbB4CTF

    Plasmid

    #17803

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pLKO.1-blast shGAC

    Plasmid

    #110410

    Purpose

    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IL2 F42A pAcGP67a

    Plasmid

    #41807

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Phenylalanine 42 to Alanine

    Promoter

    Polyhedron

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

26. Tol2-LyzC-GFP-U6ac-rac2-guides

    Plasmid

    #168241

    Purpose

    "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    rac2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pORANGE WASF1-GFP KI

    Plasmid

    #131502

    Purpose

    Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Wasf1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV Puro SCRIB P305L

    Plasmid

    #88887

    Purpose

    SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Proline to Leucine mutation @ 305th amino acid

    Promoter

    MSCV promoter

    Available since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGLS3-5xHRE-PHLDA3(wt)

    Plasmid

    #72557

    Purpose

    Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    PHLDA3 (PHLDA3 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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