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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7401 - 7450 of 10038 results

  1. pLVUT-tTR-KRAB

    Plasmid
    #11651
    Purpose
    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation
    Depositor
    Patrick Aebischer, Didier Trono
    Article
    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:
    Has Service
    Cloning Grade DNA
    Insert
    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11651-plasmid-map-sequence-id-217481

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  11. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    75146-plasmid-map-sequence-id-142065

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  12. mito-mGarnet2

    Plasmid
    #104310
    Purpose
    codes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97
    Insert
    mito-mGarnet2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104310-plasmid-map-sequence-id-225636

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  17. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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  20. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  21. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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Showing: 7401 - 7450 of 10038 results