We narrowed to 11,224 results for: NSI

1. pCA528 SPASTIN , residues 108-200

   Plasmid

   #180628

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

   Plasmid

   #75132

   Purpose

   Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   shBrd9_1061
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pGEX6P1-sr1(SIRPT1)

   Plasmid

   #75297

   Purpose

   Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 84-339
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LifeAct-mAvicFP1

   Plasmid

   #129512

   Purpose

   Expresses LifeAct-mAvicFP1 in mammalian cells

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   LifeAct-mAvicFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mAvicFP1 is AvicFP1 F64L + A206K

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_12xTEAD_luc2

   Plasmid

   #173004

   Purpose

   firefly luciferase reporter vector with 12x clustered TEAD binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   12xTEAD-luc2
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pSIL-eGFP-shACTN3

   Plasmid

   #52678

   Purpose

   shRNA against α-actinin-3 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN3 (Actn3 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA528 SPASTIN , residues 112-196

   Plasmid

   #180627

   Purpose

   Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 112-196

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_UBASH3B-1/1

   Plasmid

   #91462

   Purpose

   Protein expression and purification of human SH3 domain construct UBASH3B-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   UBASH3B-1/1 (UBASH3B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS416 Gal G294A YFP

    Plasmid

    #27448

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBeast-pAhpC-RBS- sfGFP

    Plasmid

    #190051

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. All-in-one split ADAR2(E488Q)

    Plasmid

    #170137

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid

    #104353

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Timothy Springer, Pontus Nordenfelt

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AA1-733 ITGB2 present

    Promoter

    CMV

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV7.1_3xFLAG-MOB1A

    Plasmid

    #172988

    Purpose

    constitutive expression of FLAG-tagged MOB1A

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-MOB1A (MOB1A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

    Plasmid

    #75129

    Purpose

    Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

16. RGD-sfGFP-I27-CC

    Plasmid

    #85392

    Purpose

    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCold TDP43 G294A

    Plasmid

    #27452

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. BAT-gal

    Plasmid

    #20889

    Depositor

    Stefano Piccolo

    Article

    Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

    Insert

    7XTCF binding sites, Siamois minimal promoter, NLS-lacz
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    7 multimerized TCF-binding elements cloned upstre…

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pAdEasy-hALDOA

    Plasmid

    #210812

    Purpose

    To package adenovirus expressing wild-type aldolaseA

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Syn-ChromeQ-GFP

    Plasmid

    #123317

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available since

    March 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMJA104

    Plasmid

    #128520

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme I56T (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PAmCherry1-N1-Lyn11

    Plasmid

    #202683

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    LYN(1-11):PAmCherry1 (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMA7

    Plasmid

    #79967

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. C1-FKBP(F36V):mCherry-Akt2(K14A)

    Plasmid

    #72906

    Purpose

    Recruitable Akt2(K14A) mutant

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    RAC-beta serine/threonine-protein kinase (AKT2 Human)
    

    Use

    Tags

    FKBP(F36V), mCherry

    Expression

    Mammalian

    Mutation

    K14A mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MAC_N_ARP3B

    Plasmid

    #111680

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    ARP3B (ACTR3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO.1-blast shGAC

    Plasmid

    #110410

    Purpose

    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_PIK3R2-1/1

    Plasmid

    #91415

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R2-1/1 (PIK3R2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p4XBRE-SBE-SV40-GL3

    Plasmid

    #67811

    Purpose

    luciferase reporter with mouse Id1 promoter fragments

    Depositor

    Ron Prywes

    Article

    Lewis et al Biochim Biophys Acta. 2013 Oct;1829(10):

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Id1/SV40

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

31. tdp43-EGFP construct11

    Plasmid

    #28204

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pHH0103_SHANK3-1/1

    Plasmid

    #91421

    Purpose

    Protein expression and purification of human SH3 domain construct SHANK3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SHANK3-1/1 (SHANK3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGLS3-5xHRE-PHLDA3(wt)

    Plasmid

    #72557

    Purpose

    Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    PHLDA3 (PHLDA3 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103_NOXO1-1/2

    Plasmid

    #91499

    Purpose

    Protein expression and purification of human SH3 domain construct NOXO1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NOXO1-1/2 (NOXO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-30a(+)-Syk-tSH2-PM

    Plasmid

    #111271

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y130E mutation to mimic phosphorylation

    Promoter

    T7 promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEX- HcRed -hLC3ΔG

    Plasmid

    #24992

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

39. mEmerald-H2B-6

    Plasmid

    #54111

    Purpose

    Localization: Nucleus/Histones, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pHH0103_TNK1-1/1-SV1/3

    Plasmid

    #91548

    Purpose

    Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK1-1/1-SV1/3 (TNK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge