We narrowed to 80,372 results for: TRI

1. pGEX-6P-1-HsRCK_E

   Plasmid

   #146210

   Purpose

   Bacterial Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

   Plasmid

   #202644

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   PM-mKate2-P2A-GEM-LTB4mut
   

   Use

   Tags

   PM-mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE_ENTR_CBF-GLuc_L3-L2

   Plasmid

   #113868

   Purpose

   Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CBF (notch dependent promoter)
   GLuc
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-leu-HYB

   Plasmid

   #64332

   Purpose

   leu2 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of leu2 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. p5M-hPR-B

   Plasmid

   #89114

   Purpose

   mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-B (PGR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 restriction sites deleted in polylinker

   Promoter

   CMV

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEYFP-N1-eKR2

   Plasmid

   #115337

   Purpose

   Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

   Depositor

   Peter Hegemann

   Article

   Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

   Insert

   eKR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Twitch-1 pcDNA3

   Plasmid

   #49812

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-1
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Twitch-4 pcDNA3

    Plasmid

    #49533

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-4
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-CMV-GFP-2A-G(N2c)-WPRE

    Plasmid

    #194352

    Purpose

    Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

    Depositor

    Marco Tripodi

    Article

    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    Rabies CVS-N2c glycoprotein (G)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

    Plasmid

    #113864

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    PTCH1 (hedgehog dependent promoter)
    mTurquoise2
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-E1.1-RFP

    Plasmid

    #22928

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    E1.1 binding site from Scardigli et al., 2003 with minimal CMV
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV4a-SIRT6-H133Y-FLAG

    Plasmid

    #102324

    Purpose

    Human SIRT6 H133Y with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_mGFP-FGFR3

    Plasmid

    #191752

    Purpose

    Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-Promoter-4xBS_BHLHE40

    Plasmid

    #153064

    Purpose

    Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    4X consensus BHLHE40 binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTK47_mCherry-LGN-hardened

    Plasmid

    #46347

    Purpose

    rescues the phenotypes caused by LGN RNAi

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN (GPSM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

    Promoter

    CMV

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-rTH-tTA

    Plasmid

    #133268

    Purpose

    An AAV genome that expresses tTA from the rat TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    rTH

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-Akt(K179M)

    Plasmid

    #39535

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt(K179M) (Akt1 Mouse)
    

    Use

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    K179M

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid

    #59313

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Tito Serafini, Peter Scheiffele

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. myc-BioID-Rab7

    Plasmid

    #121049

    Purpose

    Expresses myc tagged BioID and human Rab7a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Inserts

    BioID
    Rab7A
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3xFLAG-CMV14-mULK1

    Plasmid

    #24301

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pLVX Flag RagC WT

    Plasmid

    #112756

    Purpose

    express Flag-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV_nLightR

    Plasmid

    #217657

    Purpose

    Expresses norepinephrine indicator nLightR under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-hSyn-Gβγ-BERKY1

    Plasmid

    #158429

    Purpose

    BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK3ct
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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