167,601 results

1. pDsRed-Sensor

   Plasmid

   #22743

   Depositor

   Lynn Hudson

   Article

   Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

   Insert

   modified pCMV-DsRed-Express (Clontech)
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lyso-GA

   Plasmid

   #209874

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. canxTM-mEmerald (cytosolic)

   Plasmid

   #186962

   Purpose

   Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) and add ER signal…

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV5)

   Viral Prep

   #162382-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)

   Viral Prep

   #124603-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Tags

   mRuby2 (Cre-dependent)

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pCS2+N-3xHA Cloning Vector

   Plasmid

   #196653

   Purpose

   N-terminal 3xHA mammalian expression plasmid

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR47S Cm

   Plasmid

   #41892

   Depositor

   Howard Steinman

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb

   Expression

   Bacterial

   Available Since

   May 1, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TFORF1431

   Plasmid

   #143271

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF219 (ZNF219 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV2)

   Viral Prep

   #105553-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. tFucci(SA)5

    Plasmid

    #153520

    Purpose

    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

    Insert

    tFucci(SA)5.
    

    Tags

    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

    Plasmid

    #153004

    Purpose

    Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (N) (G78)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E125 Hs.MAPK1

    Plasmid

    #70409

    Purpose

    Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMS-II-dCas13-APO1-YTH

    Plasmid

    #204767

    Purpose

    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    dCas13-NLS-APO1-YTH-HA
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    Ef1alpha

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-copGFP-T2A-Puro

    Plasmid

    #72263

    Purpose

    Express copGFP and puromycin resistance gene under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    elongation factor-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. ABE8e-NG

    Plasmid

    #226856

    Purpose

    Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIneo RYR-1

    Plasmid

    #75113

    Purpose

    Clone encoding rabbit ryanodine receptor in mammalian expression vector

    Depositor

    Robert Dirksen, Paul Allen

    Article

    Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

    Insert

    Ryanodine receptor 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0883

    Plasmid

    #141749

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTRIPZ-hDDX5/17

    Plasmid

    #71307

    Purpose

    Inducible shRNA knockdown of both human DDX5 and DDX17 genes

    Depositor

    Allan Brasier

    Article

    Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

    Insert

    DDX5/17 shRNA (DDX17 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJMP1360

    Plasmid

    #119278

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFUSEss-CHIg-mM_M18

    Plasmid

    #91729

    Purpose

    Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Expression

    Mammalian

    Promoter

    hEF1-HTLV prom

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. slc1a3b:myrGFP-P2A-H2AmCherry

    Plasmid

    #170208

    Purpose

    Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGFP-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGME

    Plasmid

    #71252

    Purpose

    luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF enhancer (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pExp-NHis

    Plasmid

    #112558

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162233

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody lag16lag2 and IFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. RF2

    Bacterial Strain

    #62070

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

30. CDC34

    Plasmid

    #25598

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDC34 (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pMRX-IP-EGFP-mouse Plaat3 (WT)

    Plasmid

    #167987

    Purpose

    Expresses EGFP-Plaat3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LeGO-V2

    Plasmid

    #27340

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Venus fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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35. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Human ubiquitination-related proteins CRISPR KO library

    Pooled Library

    #174592

    Purpose

    CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.

    Depositor

    Juan Bonifacino

    Article

    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

37. Flag-HA-USP33

    Plasmid

    #22601

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP33 (USP33 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MBP-FUS_FL_WT

    Plasmid

    #98651

    Purpose

    Expresses MBP-tagged full length FUS

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMV-SB100X[EF1α-copGFP]

    Plasmid

    #218323

    Purpose

    Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge