Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,890 results

Showing: 6201 - 6240 of 164890 results

  10. JD633

    Plasmid
    #160393
    Purpose
    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning
    Depositor
    Jorge Dubcovsky
    Article
    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103
    Inserts
    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    Use
    Binary vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160393-plasmid-map-sequence-id-456253

    Enlarge

  12. TFORF3117

    Plasmid
    #144593
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MZF1 (MZF1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144593-plasmid-map-sequence-id-404292

    Enlarge

  16. p405MET25

    Plasmid
    #15961
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restricti…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15961-plasmid-map-sequence-id-8446

    Enlarge

  17. pAAV.CMV.ffLuciferase.SV40 (AAV8)

    Viral Prep
    #105532-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.ffLuciferase.SV40 (#105532). In addition to the viral particles, you will also receive purified pAAV.CMV.ffLuciferase.SV40 plasmid DNA. CMV-driven ffLuciferase control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    ffLuciferase
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. pTA7001-DEST

    Plasmid
    #71745
    Purpose
    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible
    Depositor
    Roger Innes
    Article
    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    GAL4
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71745-plasmid-map-sequence-id-136974

    Enlarge

  25. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

    Enlarge

  29. TMED9-bio-His

    Plasmid
    #51855
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED9 (TMED9 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51855-plasmid-map-sequence-id-79742

    Enlarge

  32. pZac2.1-GfaABC1D-lck-jGCaMP8f (AAV9)

    Viral Prep
    #176759-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8f (#176759). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8f plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    GfaABC1D
    Tags
    None
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  34. TFORF0219

    Plasmid
    #142563
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ADNP (ADNP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142563-plasmid-map-sequence-id-395965

    Enlarge

  38. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

    Enlarge

  40. pAAV-hSyn-EGFP (AAV9 trial size)

    Viral Prep
    #50465-AAV9.T
    Purpose
    Ready-to-use AAV9 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 6201 - 6240 of 164890 results