163,297 results

1. pDSG290

   Plasmid

   #115609

   Purpose

   surface-displayed Nb3, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV Luciferase PGK-hygro

   Plasmid

   #18782

   Depositor

   Scott Lowe

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDSG288

   Plasmid

   #115607

   Purpose

   surface-displayed Ag3, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQCXIP-GFP-RPA70

   Plasmid

   #164231

   Purpose

   retroviral plasmid for GFP-RPA70

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   RPA70 (RPA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Red cGull

   Plasmid

   #191853

   Purpose

   Expresses biosensor for cGMP in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

   Insert

   Red cGull
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Rab5B

   Plasmid

   #61802

   Purpose

   Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   RAB5B (RAB5B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CXCR7-DuET

    Plasmid

    #213224

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR7 (ACKR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-tdMCP-YFP

    Plasmid

    #99151

    Purpose

    Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    Dimeric MS2-coat binding protein
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_SFFv_Her2-BFP_RHL027

    Plasmid

    #164823

    Purpose

    Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. STICR_Index1

    Pooled Library

    #186334

    Purpose

    High Complexity Lentiviral Barcode Library

    Depositor

    Tomasz Nowakowski

    Article

    Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

17. pH2B-emiRFP670

    Plasmid

    #136571

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP670
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

    Plasmid

    #192636

    Purpose

    Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Brainbow3.0

    Plasmid

    #45176

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pCAG-Brainbow3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    Lambda-PPase
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EFSp-GFP-YAP (5SA)

    Plasmid

    #174170

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    EFS

    Available since

    Sept. 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha/TRE3G

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (AAV PHP.eB)

    Viral Prep

    #214856-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (#214856). In addition to the viral particles, you will also receive purified AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) plasmid DNA. minBG-driven expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Promoter

    Tags

    None

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-ExRai-AktAR2

    Plasmid

    #184047

    Purpose

    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-opto-hCaspase-8

    Plasmid

    #208786

    Purpose

    Inducibly expresses human Caspase 8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase 8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I319V, N451D, D532N

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSET.HaloTag.v7

    Plasmid

    #226197

    Purpose

    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1 hygro shRNA E-cadherin

    Plasmid

    #18802

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-neo-cterminal-3HA

    Plasmid

    #102643

    Purpose

    To generate three copies of the HA epitope tag on cDNAs.

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    3x HA (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmGFP-P2A-K(AAA)20-P2A-RFP

    Plasmid

    #105688

    Purpose

    Double fluorescent stalling reporter K(AAA)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)20
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SigmaR1-mNeonGreen

    Plasmid

    #226566

    Purpose

    Encodes SigmaR1 protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. tetO-FOSL1

    Plasmid

    #170687

    Purpose

    doxycycline-inducible overexpression of FOSL1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid

    #12252

    Purpose

    3rd generation lentiviral backbone

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge