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171,789 results

Showing: 7801 - 7850 of 171789 results

  6. pTCF200 Mouse beta-2 microglobulin

    Plasmid
    #180462
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Mouse beta-2 microglobulin
    Tags
    BSP41
    Expression
    Bacterial
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180462-plasmid-map-sequence-id-355083

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  10. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  14. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  16. pTCF120 H-2D(b)

    Plasmid
    #180454
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2D(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180454-plasmid-map-sequence-id-355842

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  20. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  25. pRS415-GPD

    Plasmid
    #183940
    Purpose
    Control vector that has a GPD promoter with no insert
    Depositor
    Michael Downey
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183940-plasmid-map-sequence-id-363367

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  27. pAAV-S5E2-dTom-nlsdTom (AAV1)

    Viral Prep
    #135630-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    dTomato
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  32. CECR2

    Plasmid
    #39066
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CECR2 (CECR2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid
    #135241
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135241-plasmid-map-sequence-id-268090

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  37. TFORF2558

    Plasmid
    #144043
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TLX1 (TLX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144043-plasmid-map-sequence-id-400565

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  44. pAAV-CAG-H2B-GFP (AAV Retrograde)

    Viral Prep
    #116869-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-H2B-GFP (#116869). In addition to the viral particles, you will also receive purified pAAV-CAG-H2B-GFP plasmid DNA. CAG-driven expression of H2B-GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1
    Tags
    GFP
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (AAV8)

    Viral Prep
    #65417-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (#65417). In addition to the viral particles, you will also receive purified pAAV-hSyn-dF-HA-KORD-IRES-mCitrine plasmid DNA. hSyn-driven, Cre-dependent KORD expression with bicistronic mCitrine, for SALB-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)
    Promoter
    Syn
    Tags
    mCitrine
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only

  49. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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Showing: 7801 - 7850 of 171789 results