Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

167,093 results

Showing: 7801 - 7850 of 167093 results

  4. pRSF-G1mCNPRS

    Plasmid
    #174718
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174718-plasmid-map-sequence-id-346623

    Enlarge

  6. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67513-plasmid-map-sequence-id-381535

    Enlarge

  12. p20StAD

    Plasmid
    #190268
    Purpose
    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.
    Depositor
    Assaf Mosquna
    Article
    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2
    Type
    Empty backbone
    Use
    Synthetic Biology; Yeast genome-integrating vector
    Tags
    GAL4-AD
    Expression
    Mutation
    Promoter
    TDH3
    Available Since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    190268-plasmid-map-sequence-id-377252

    Enlarge

  17. pSOX11-donor

    Plasmid
    #176360
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    SOX11 (SOX11 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176360-plasmid-map-sequence-id-347860

    Enlarge

  21. LV-miR-142

    Plasmid
    #117056
    Purpose
    Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression
    Depositor
    Michele De Palma
    Article
    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d
    Insert
    miR-142-3p - OFP (Mir142 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFV promoter
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117056-plasmid-map-sequence-id-223999

    Enlarge

  22. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

    Enlarge

  26. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid
    #148120
    Purpose
    Mammalian Expression of HshnRNPH1
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148120-plasmid-map-sequence-id-396934

    Enlarge

  27. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid
    #170196
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    Use
    Tags
    3C-His8-Strep2x2
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…
    Promoter
    CMV51
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    170196-plasmid-map-sequence-id-334281

    Enlarge

  31. XX01CAPN2A-c001

    Plasmid
    #175492
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN2 / CAPNS1 (CAPN2 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175492-plasmid-map-sequence-id-353163

    Enlarge

  35. pSOX11.1.0-gDNA

    Plasmid
    #176378
    Purpose
    Expresses spCas9 and gRNA sequence targeting SOX11
    Depositor
    Kevin White, Barbara Stranger
    Insert
    SOX11 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176378-plasmid-map-sequence-id-347389

    Enlarge

  43. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

    Viral Prep
    #171027-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Patricia Jensen
    Article
    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi
    Promoter
    EF1a
    Tags
    tdTomato
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  49. pAAV_BiLAMP5e3_dTomato_nlsdTomato (AAV PHP.eB)

    Viral Prep
    #218792-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiLAMP5e3_dTomato_nlsdTomato (#218792). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the Lamp5 interneuron-targeting enhancer. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Promoter
    Tags
    None
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 7801 - 7850 of 167093 results