We narrowed to 17,478 results for: IGH@

Showing: 7901 - 7950 of 17478 results

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Rabenosyn-5

Plasmid

#37538

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pIVT-eeBxb1

Plasmid

#232748

Purpose

IVT template for making modRNA encoding eeBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A, E229K, V375I

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA2.0

Plasmid

#158394

Purpose

Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-mTGFB1

Plasmid

#219553

Purpose

mTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

mTGFB1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ133-tRNA2.0

Plasmid

#158395

Purpose

Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR1-SV40-puro

Plasmid

#240535

Purpose

Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SpCas9-CBE6a

Plasmid

#215819

Purpose

Express CBE6a (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM5-N-22

Plasmid

#54170

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM5

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-MCM7-N-22

Plasmid

#54174

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM7

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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LentiV2-EF1α-nCas9/RT

Plasmid

#210188

Purpose

Expression of nCas9/RT-P2A-Puro

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

nCas9/RT-P2A-Puro

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCC5)

Plasmid

#235073

Purpose

Expresses Tad-CBE(CCC5) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC5)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSIQ

Plasmid

#216238

Purpose

All-in One IQ transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-DuraPETase

Plasmid

#218699

Purpose

Encodes codon optimized DuraPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DuraPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Flp-2A-BleoR

Plasmid

#232752

Purpose

IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Inserts

FlpO
BleoR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GFP-Dub3 mouse

Plasmid

#72683

Purpose

mammalian expression of GFP-Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-EGFP barcode-5-SV40 polyA

Plasmid

#190869

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode5

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT-iCre

Plasmid

#232773

Purpose

IVT template for making modRNA encoding iCre

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

iCre

Use

Cre/Lox; In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-cMSP1E3D1

Plasmid

#199772

Purpose

Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

Insert

cMSP1E3D1

Tags

His6-Tag

Expression

Bacterial

Mutation

Extended cMSP1D1; external His-Tags flanking the …

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLR2070

Plasmid

#28063

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Contains N496D point mutation in FokI domain, as …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLR2068

Plasmid

#28062

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Contains H537R point mutation in FokI domain, as …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-Lifeact_Halo_T2A_EGFP

Plasmid

#135445

Purpose

Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

Lifact-HaloTag7 and EGFP

Tags

Lifact

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HcKCR2_mCherry_pcDNA3.1

Plasmid

#177337

Purpose

Expresses KCR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Salsa-BC1-P1A1

Plasmid

#202842

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC1

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK346 (Hygro-P2A-mAID-mCherry)

Plasmid

#121180

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mCherry

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(CCC2)

Plasmid

#235072

Purpose

Expresses Tad-CBE(CCC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SpRY-Tad-CBE(ACC2)

Plasmid

#235081

Purpose

Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSSBIO32_dCas9_RPA1786

Plasmid

#240489

Purpose

CRISPRi-mediated Knockdown of RPA1786

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-mCherry-XL (R125K)

Plasmid

#188054

Purpose

Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

6xHis

Expression

Bacterial

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

araBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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