We narrowed to 18,888 results for: REV

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pcDNA-Wnt2

Plasmid

#35906

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pNC1011

Plasmid

#42898

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

3 tandem copies of GFP*

Use

Yeast integrating

Mutation

changed Phenylalanine 64 to Leucine, Serine 65 t…

Promoter

None

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS7

Plasmid

#111761

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS7-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-L2T3D

Plasmid

#33277

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L2 (L2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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9xHRE-MinTK-CRE

Plasmid

#141146

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

9xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDH42(CUG/A)

Plasmid

#154132

Purpose

Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

engineered bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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3539 pMIG Bax

Plasmid

#8788

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bax (Bax Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pAAV2-GCaMP6f-P2A-Vmn1r237

Plasmid

#133417

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 237

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r237 (Vmn1r237 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXFokI-dCas9

Plasmid

#60901

Purpose

Dual Expression Vector for FokI-dCas9 and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-NUDT5

Plasmid

#219954

Purpose

For gateway cloning of NUDT5

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

NUDT5 (NUDT5 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PTK2-C-10

Plasmid

#55441

Purpose

Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PTK2 (PTK2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
HA-mEOS3.2-TRF2 HR Donor

Plasmid

#86845

Purpose

HR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

HA-mEOS3.2-TRF2 at endogenous locus (TERF2 Human)

Tags

HA-mEOS3.2

Expression

Mammalian

Promoter

Endogenous TRF2 promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-L1T1L

Plasmid

#33286

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8A

Plasmid

#35916

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Expression

Mammalian

Mutation

Silent Mutation A (600) --> G

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r78

Plasmid

#133413

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 78

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GST-RFP-cb5

Plasmid

#137754

Purpose

Targets red flourescent protein (RFP) to the endoplasmic reticulum

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

RFP-cb5

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-S4T3D

Plasmid

#33285

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S4 (S4 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-122

Plasmid

#26325

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-122

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-M1T1L

Plasmid

#33289

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M1 (M1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
SHC003BSD

Plasmid

#133300

Purpose

The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

Depositor

Jingshi Shen

Article

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-GCaMP6f-P2A-Vmn1r86

Plasmid

#133415

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CALCOCO1 W47A-mycDDK

Plasmid

#137769

Purpose

CALCOCO1 LIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 W47A

Tags

mycDDK

Expression

Mammalian

Mutation

W47A

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21(+)-His6-Ubc13

Plasmid

#212715

Purpose

Bacterial expression of His6-Ubc13

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-M3T3D

Plasmid

#33281

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M3 (M3 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-S2T3D

Plasmid

#33283

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S2 (S2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-L3T3D

Plasmid

#33278

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L3 (L3 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-M1T3D

Plasmid

#33279

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M1 (M1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-M2T1L

Plasmid

#33290

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M2 (M2 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mTubulin NLS-mCherry-LEXY

Plasmid

#182597

Purpose

Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

mCherry

Tags

LEXY and NLS

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_DMS3g24

Plasmid

#89244

Purpose

Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

CRISPR

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
e-ciCas9_pcDNA5

Plasmid

#100553

Purpose

Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

e-ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF257

Plasmid

#52533

Purpose

expresses I-SceI nuclease in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

I-SceI (SCEI Budding Yeast)

Tags

HA and NLS

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-S3T3D

Plasmid

#33284

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S3 (S3 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-M2T3D

Plasmid

#33280

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

M2 (M2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-ySet2

Plasmid

#51698

Purpose

Expresses full length yeast Set 2 gene in a mamalian expression vector

Depositor

Tom Misteli

Article

Luco et al Science. 2010 Feb 19;327(5968):996-1000.

Insert

yeast Set2 (SET2 Budding Yeast)

Tags

C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only
rat APOBEC1

Plasmid

#112858

Purpose

plasmid for expression of rat APOBEC1 in mammalian cells

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDYSCKO-ADE1

Plasmid

#120264

Purpose

pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

pDYSCKO cassette-ADE1 targeting guide

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AL13Rb-GAPC2

Plasmid

#161009

Purpose

PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-GPD-VPH2 chr I

Plasmid

#41897

Purpose

Yeast expression of VPH2.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VPH2 (VPH2 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1539

Plasmid

#111434

Purpose

Genedrive entry vector for Candida albicans

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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