We narrowed to 32,508 results for: Eng;

1. pCBFRE-luc

   Plasmid

   #26897

   Depositor

   Nicholas Gaiano

   Article

   Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

   Insert

   CBFRE
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCM271rfp

   Plasmid

   #59774

   Purpose

   pCMrfp with TrfA mutation (271R to C)

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   RFP
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Tags

   his tag and sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-myc-Wnk1

   Plasmid

   #38779

   Depositor

   Melanie Cobb

   Article

   Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

   Insert

   Wnk1 (Wnk1 Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCM4.4

   Plasmid

   #217648

   Purpose

   an ermE*p-cas9-driven expression, as an alternative plasmid

   Depositor

   Huimin Zhao

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   Codon Optimized SpCas9
   

   Use

   CRISPR

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-BRIPO

   Plasmid

   #215542

   Purpose

   BRIPO (Bioluminescent Red Indicator for Potassium)

   Depositor

   Huiwang Ai

   Article

   Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

   Insert

   BRIPO
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMIN-Ri

   Plasmid

   #197784

   Purpose

   Level 2 destination vector: pMIN-Ri backbone

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-EFS-PCP-3XNeon-nls

   Plasmid

   #75386

   Purpose

   PCP-3XNeon-nls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   3XmNeonGreen

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_CnCas12f1

   Plasmid

   #204996

   Purpose

   CnCas12f1 heterologous expression plasmid

   Depositor

   Quanjiang Ji

   Article

   Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

   Insert

   CnCas12f1
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLH-sgRNA1-2XboxB

    Plasmid

    #75391

    Purpose

    sgRNA1-2XboxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XboxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-MSCV-4xS1m-GFP-T2A-PU

    Plasmid

    #138581

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    S1m tag

    Promoter

    MSCV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCWori-A13AMO-aaCPRct

    Plasmid

    #20117

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    Amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcS-p88-C1C2

    Plasmid

    #178424

    Purpose

    p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    p88
    

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His_CL7_2NLS_iCas12a-4NLS

    Plasmid

    #222971

    Purpose

    Bacterial expression of NLS-rich iCas12a construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His_CL7_2NLS_iCas12a-4NLS
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pV1090

    Plasmid

    #111427

    Purpose

    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEVA13-GG

    Plasmid

    #208002

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAd-PV-NLS-DsRed

    Plasmid

    #17287

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS-CD (Pvalb Rat)
    

    Use

    Adenoviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

20. DV4 VLPs pVRC8400

    Plasmid

    #63164

    Purpose

    DV4 prM-E structural cassette in pVRC8400

    Depositor

    Stephen Harrison

    Article

    Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

    Insert

    DV4 prM-E
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SPS-7942

    Plasmid

    #80459

    Purpose

    IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

    Depositor

    Daniel Ducat

    Article

    Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

    Insert

    Sucrose phosphate synthase
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA 3.1(-) VSFP3.1

    Plasmid

    #18951

    Depositor

    Thomas Knopfel

    Article

    Lundby et al PLoS ONE. 2008 . 3(6):e2514.

    Insert

    VSFP3.1
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAd-PV-NES-DsRed

    Plasmid

    #17286

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-NES (Pvalb Rat)
    

    Use

    Adenoviral

    Tags

    DSR and Myc

    Expression

    Mammalian

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pKD182

    Plasmid

    #90952

    Purpose

    Expresses ThsS under the Ptac promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    ThsS
    

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. wt HIV-1 integrase in pET15b

    Plasmid

    #61668

    Purpose

    wild type HIV-1 integrase in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    wild type HIV-1 integrase
    

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pREP

    Plasmid

    #51491

    Purpose

    pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Rep/RepA
    

    Use

    Plant expression t-dna

    Mutation

    none

    Promoter

    2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Opto-hEGFR_308

    Plasmid

    #58746

    Purpose

    Expresses Opto-hEGFR (light-activated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    Opto-hEGFR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DV3 VLPs pVRC8400

    Plasmid

    #63163

    Purpose

    DV3 prM-E structural cassette in pVRC8400

    Depositor

    Stephen Harrison

    Article

    Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

    Insert

    DV3 prM-E
    

    Expression

    Mammalian

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAL-pck_T

    Plasmid

    #74068

    Purpose

    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pET29-neoLux1.2

    Plasmid

    #229952

    Purpose

    Bacterial expression for de novo luciferase, neoLux1.2

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    neoLux1.2
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWS931

    Plasmid

    #123053

    Purpose

    TetR bacterial repressor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    TetR-NLS [3a]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDM344

    Plasmid

    #108974

    Purpose

    shuttle vector

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum shuttle vector

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSB1C3-J23110-B0034-mRFP1_Violet

    Plasmid

    #160447

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Violet
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNC-Cam1304-MCS

    Plasmid

    #193387

    Purpose

    Nimble cloning-compatible vector for plant ectopic expression

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pWS1806

    Plasmid

    #123047

    Purpose

    Melatonin GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    MTNR1A [3]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pExchange_tdk_BT1311p-tetR-2

    Plasmid

    #90326

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWS625

    Plasmid

    #123046

    Purpose

    Adenosine GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    A2BR [3]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMVA

    Plasmid

    #121149

    Purpose

    This plamid was used for improving the production of DMAPP (IPP) in E.coli

    Depositor

    Minjuan Xu

    Article

    He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

    Insert

    MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
    

    Use

    Synthetic Biology

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBT224_(pCA-tTA2)

    Plasmid

    #36430

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tetracycline transactivator
    

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME-TagBFP2-NS

    Plasmid

    #80502

    Purpose

    Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    TagBFP2 (no STOP)
    

    Use

    Multisite gateway middle entry clone

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJMP8045

    Plasmid

    #220900

    Purpose

    Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA5/FRT/TO_Lifeact_HaloTag7

    Plasmid

    #175535

    Purpose

    Expression of HaloTag7 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mV-NcVV-LOV_231

    Plasmid

    #58689

    Purpose

    Expresses mVenus-fusion protein of NcVV-LOV domain (Neurospora crassa Vivid)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-NcVV-LOV
    

    Expression

    Mammalian

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pYPQ133-tRNA

    Plasmid

    #179212

    Purpose

    Golden Gate entry vector; 3rd gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    no promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. VV063: 1xCox8 - superecliptic pHluorin in fck

    Plasmid

    #58500

    Purpose

    Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Superecliptic pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRK DPC4 Flag (aka: Smad4 Flag)

    Plasmid

    #12627

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

    Insert

    DPC4 (SMAD4 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

48. pSB1C3-J23110-B0034-mRFP1E

    Plasmid

    #160442

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA5/FRT/TO_NES_HaloTag7

    Plasmid

    #175527

    Purpose

    Strict cytosolic expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pENTR APP shRNA 1

    Plasmid

    #30134

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP shRNA 1
    

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only