We narrowed to 32,508 results for: Eng;

1. BSP610

   Plasmid

   #122255

   Purpose

   expression vector for mtagBFP2 under EFT3 promoter

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   mtagBFP2
   

   Expression

   Worm

   Promoter

   EFT3

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEntr_PE2

   Plasmid

   #149548

   Purpose

   Gateway entry clone with PE2 enzyme (with stop codon)

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR; Gateway shuttle vector

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNC-Green-LUC

   Plasmid

   #193438

   Purpose

   Nimble cloning-compatible vector for the analysis of promoter activity in plants

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_LAMP1_HaloTag7

   Plasmid

   #175531

   Purpose

   Lyzosomal expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag7
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJG/ Inducible ALPHA MHC

   Plasmid

   #55792

   Purpose

   transgene expression driven by inducible ALPHA MHC promoter

   Depositor

   Jeffrey Robbins

   Article

   Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   Inducible ALPHA MHC

   Available Since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

6. VV064: 1xCox8 - ratiometric pHluorin in fck

   Plasmid

   #58502

   Purpose

   Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - Ratiometric pHluorin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Promoter

   vasa

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pYPQ141B-ge4.1

   Plasmid

   #205144

   Purpose

   Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

   Depositor

   Yiping Qi

   Article

   Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Promoter

   AtU3

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGlnA-Ypet

    Plasmid

    #98278

    Purpose

    mammalian expression of GlnA-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    GlnA
    

    Tags

    Ypet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAL-pck_NT

    Plasmid

    #74069

    Purpose

    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (NT)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pFtnA-Halo

    Plasmid

    #98281

    Purpose

    mammalian expression of FtnA-Halo

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    FtnA
    

    Tags

    Halo-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR1a-MTS-jAspSnFR3

    Plasmid

    #203461

    Purpose

    Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3
    

    Use

    Gateway entry vector

    Tags

    Mitochondrial localization signal

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMTU-DO-URA-C4

    Plasmid

    #160339

    Purpose

    High-copy expression vector for K. marxianus, uracil selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUSE-RapR-Src-myc

    Plasmid

    #25933

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-Src (Src Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Expression

    Insect

    Promoter

    Act5c

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfRΔEctoR1-mRFP-GFPnb

    Plasmid

    #113555

    Purpose

    Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    TfRΔEctoR1-mRFP-GFPnb
    

    Expression

    Mammalian

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ134-tRNA

    Plasmid

    #179213

    Purpose

    Golden Gate entry vector; 4th gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    no promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EphA1-RTK-Cry2olig

    Plasmid

    #176601

    Purpose

    EphrinA1 RTK fusion to Cry2olig - RTK domain only

    Depositor

    Robert Hughes

    Article

    Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

    Insert

    EphA1rtk-Cry2olig-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pREPK219H

    Plasmid

    #51492

    Purpose

    pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Rep/RepA
    

    Use

    Plant expression t-dna

    Mutation

    changed lysine 219 to histidine

    Promoter

    2x35S

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET22-HT/spoR

    Plasmid

    #73631

    Purpose

    Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    guanylate kinase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWS2247

    Plasmid

    #123026

    Purpose

    Tet promoter (TetO(x7)-pLEU2m)

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pTet [2]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSMVP-Cas9N

    Plasmid

    #80934

    Purpose

    Plasmid that expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCM291rfp

    Plasmid

    #59775

    Purpose

    pCMrfp with TrfA mutation (291E to G)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGT-Ah5

    Plasmid

    #122572

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LP538

    Plasmid

    #90202

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC8 full length

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC8 (TRAPPC8 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET51b_SNAP-ecDHFR

    Plasmid

    #187108

    Purpose

    Expression of SNAP-ecDHFR fusion in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    SNAP-ecDHFR
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-51b-HaloTag10

    Plasmid

    #175520

    Purpose

    Expression of HaloTag10 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag10
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAM282

    Plasmid

    #226121

    Purpose

    Expression of cdc48

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    His6-3C-Cdc48 (CDC48 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PSICORhp-mp53

    Plasmid

    #26273

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

    Insert

    p53 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pSMVP-Cas9C

    Plasmid

    #80939

    Purpose

    Plasmid that expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5/FRT/TO_HaloTag7_SKL

    Plasmid

    #175532

    Purpose

    Peroxysomal expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-SKL
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSB1C3-J23110-B0034-mRFP1_Magenta

    Plasmid

    #160446

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Magenta
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. rOpto-rtrkB_397

    Plasmid

    #82681

    Purpose

    Expresses rOpto-rtrkB (light-activated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

    Insert

    rOpto-rtrkB
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGT-Ah3

    Plasmid

    #122570

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHR_EF1a-->myc-mtC11L34

    Plasmid

    #162226

    Purpose

    Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-PNE scFv C11L34
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. vgCam-pRSETb

    Plasmid

    #193362

    Purpose

    A FRET-based calcium probe using Sumire

    Depositor

    Takeharu Nagai

    Article

    Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

    Insert

    vgCam
    

    Use

    E.coli expression

    Tags

    His X6 tag and Strep tag

    Promoter

    T7

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKM492-Myc-His

    Plasmid

    #109283

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Myc-His
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHCKAn-phoAp-GRFT

    Plasmid

    #158747

    Purpose

    low phosphate phoAp induction of Griffithsin

    Depositor

    Michael Lynch

    Article

    Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

    Insert

    phoAp Griffithsin
    

    Expression

    Bacterial

    Promoter

    phoA

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYPQ151

    Plasmid

    #69302

    Purpose

    Gateway entry vector with pcoCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9D10A (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWS926

    Plasmid

    #123052

    Purpose

    LexA bacterial repressor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexA-NLS [3a]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBC2103-reci

    Plasmid

    #117172

    Purpose

    U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_arCas9 without NLS
    

    Use

    Lentiviral

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BSP611

    Plasmid

    #122256

    Purpose

    expression vector for mtagRFP-T under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagRFP-T
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHR_TRE3GS-->GssGFP-P2A-NLSIFP

    Plasmid

    #162235

    Purpose

    Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    GFP - IFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNC-TRV2

    Plasmid

    #193402

    Purpose

    Nimble cloning-compatible vector for virus-induced gene silencing

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pNC-BiFC-ED1

    Plasmid

    #193426

    Purpose

    Nimble cloning-compatible vector for plant BiFC

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA5-TO-PV-NLS-Dsred

    Plasmid

    #16341

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS (Pvalb Rat)
    

    Tags

    Myc and NLS

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

49. pAG506-mito-frFAST

    Plasmid

    #160057

    Purpose

    Expresses mito-frFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

    Insert

    Mito-frFAST
    

    Expression

    Mammalian

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDUET-ctCPR-A17AMO

    Plasmid

    #20115

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and amorphadiene oxidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only