We narrowed to 25,959 results for: Vit;

1. pLL7.0: Venus-oLID-CAAX (from KRas4B)

   Plasmid

   #60412

   Purpose

   Mammalian Expression of Venus-oLID-CAAX

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-oLID-CAAX
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5 FRT/TO MycLAP-STIL ∆CC

   Plasmid

   #80268

   Purpose

   mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   STIL (STIL Human)
   

   Use

   Tags

   Myc-GFP

   Expression

   Mammalian

   Mutation

   missing conserved coiled-coil (amino acids 721-74…

   Promoter

   CMV

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-DR1-NRSE-RFP

   Plasmid

   #22927

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MS2-adRNA (3'UAG)

   Plasmid

   #170128

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(3'UAG)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFP86

   Plasmid

   #83543

   Purpose

   Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   RPL4A promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPL4A

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46593

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-16 Decoy (Mir16-1 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pENTR-GFP-Patronin CC+CKK

   Plasmid

   #59048

   Purpose

   for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pENTR-GFP-Patronin CC 534–1457

   Plasmid

   #59050

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGex-3x Flag MKK3

    Plasmid

    #14814

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK3 (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS316-RGR-GFP-mm

    Plasmid

    #51059

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pG.Lantern-SM50-GFP

    Plasmid

    #135593

    Purpose

    Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    SM50 promoter (SM50 S. purpuratus)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hNPPC-RFP

    Plasmid

    #22921

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

    Plasmid

    #163610

    Purpose

    Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWP1_mFTCD

    Plasmid

    #102317

    Purpose

    expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Ftcd (Ftcd Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS316-RGR-GFP-mHDV

    Plasmid

    #51058

    Purpose

    Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTetR-BCD2-Ana GvpC

    Plasmid

    #153298

    Purpose

    Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. D92_Ld_360170

    Plasmid

    #135905

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pM117: VEGFA array presgRNA

    Plasmid

    #166869

    Purpose

    U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting array presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-ChroME(F243Y)-mRuby2-ST

    Plasmid

    #109127

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(F243Y)-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 8xCTS1_BpiI-cloning-site_SV40polyA

    Plasmid

    #124547

    Purpose

    Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCASP-SptP120-NS1-HilA

    Plasmid

    #153327

    Purpose

    Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-NS1 Monobody
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSFFV-Cx43

    Plasmid

    #69442

    Purpose

    mammalian expression of rat Cx43

    Depositor

    Richard Veenstra

    Article

    Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

25. AV4-EF1-GFP

    Plasmid

    #124971

    Purpose

    Control for AV4-EF1-NMNAT2

    Depositor

    Kazuhiro Oka

    Article

    Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPK1E-2+t

    Plasmid

    #48148

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ABE8e(TadA-8e V82G)

    Plasmid

    #138494

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V82G)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V82G

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKDsgRNA-malG

    Plasmid

    #89951

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    malG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-h-mmPtch1-B-HA

    Plasmid

    #120902

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-HA-mIFITM3-C105A

    Plasmid

    #58392

    Purpose

    Expressed murine IFITM3-C105A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteine 105 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pKDsgRNA-hisG

    Plasmid

    #89954

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    hisG gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAGM_Puro_shRNA n.2_mTet3

    Plasmid

    #79625

    Purpose

    Expression of shRNA for mouse Tet3

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet3 (Tet3 Mouse)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pAGM_Puro_shRNA n.1_mTet3

    Plasmid

    #79624

    Purpose

    Expression of shRNA for mouse Tet3

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet3 (Tet3 Mouse)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pmCherry-Akt2(K14A)

    Plasmid

    #86624

    Purpose

    Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2(K14A) (AKT2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXPR_BRD003 sgLacZ-1

    Plasmid

    #74968

    Purpose

    sgLacZ-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pXPR_BRD003 sgLacZ-2

    Plasmid

    #74969

    Purpose

    sgLacZ-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFLAG-4EHP(K121/130/134/222R)

    Plasmid

    #17353

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    4EHP (EIF4E2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K121/130/134/222R

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pET-FLAG-eSpCas9

    Plasmid

    #126769

    Purpose

    Expression of increased fidelity eSpCas9 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pES005 (pTet-qacR wt/pQacA-deGFP)

    Plasmid

    #69034

    Purpose

    reporter with wild-type qacR on single plasmid

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Inserts

    qacR (qacR Synthetic)
    deGFP
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    pQacA and pTet

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge