We narrowed to 47,373 results for: vit;

1. p-mCherry-N1-ChloC

   Plasmid

   #85461

   Purpose

   Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Peter Hegemann

   Article

   Wietek et al Science. 2014 Mar 27.

   Insert

   ChloC
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E90R, T159C

   Promoter

   CMV (+enhancer)

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN3P_T3_ABEmax_IVT

   Plasmid

   #171761

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   ABEmax
   

   Use

   Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Gateway-DSCP

   Plasmid

   #71506

   Purpose

   flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq validation vector

   Tags

   luciferase

   Expression

   Insect

   Available Since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT44c

   Plasmid

   #138511

   Purpose

   P2

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   inteinC-dSpCas9-UGI
   

   Use

   Synthetic Biology

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AB.pCCL.sin.cPPT.U6.miR-335-5p-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46612

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-335-5p Decoy
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-mCherry-2xEcMTS

   Plasmid

   #133624

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherry-2xEcMTS
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRB-ECFP(W66A)-Giantin

   Plasmid

   #67903

   Purpose

   Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   FRB-ECFP(W66A)-Giantin
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18-H1 RNAi

    Plasmid

    #87355

    Purpose

    RNAi expression vector based on H1 promoter and pUC18 vector backbone

    Depositor

    Saroj Mathupala

    Article

    Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

    Insert

    H1 RNA Promoter from U87MG glioma cells
    

    Use

    RNAi; Shrna expression vector

    Expression

    Mammalian

    Promoter

    H1 RNA

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-hSyn-Galpha-i3 ONE-GO

    Plasmid

    #204334

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFAP-Lyn-mCherry-pHluorin

    Plasmid

    #187980

    Purpose

    Membrane targeted cytoplasmic fluorescent pH sensor

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Lyn-mcherry-pHluorin
    

    Use

    AAV

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEG R2-L3 #BGV193

    Plasmid

    #48952

    Purpose

    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. SG3ΔENV CA M66I

    Plasmid

    #149687

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residue Met 66 to Iso (CA M66I)

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46638

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-92a Decoy
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Lck-FRB-ECFP(W66A)

    Plasmid

    #67902

    Purpose

    Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    Lck-FRB-ECFP(W66A)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRN3P_T3_BE4_IVT

    Plasmid

    #171762

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE4
    

    Use

    Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-puro-TTF-1-wt

    Plasmid

    #74990

    Purpose

    Retroviral vector holding the human wild-type full-length TTF-1 (NKX2-1) cDNA

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    TTF-1 (TTF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without 3' UTR

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV4a-SIRT6-G60A-FLAG

    Plasmid

    #102323

    Purpose

    Human SIRT6 G60A with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 60 to alanine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-GBP6-10gly-VPminx4

    Plasmid

    #50796

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-VPminx4 activation domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. p3E-mTagBFP-CAAX

    Plasmid

    #75176

    Purpose

    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV3Tag8li-hSAPS1-HA

    Plasmid

    #165466

    Purpose

    Human SAPS1 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-rTetRDBD-GBP1

    Plasmid

    #50791

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    rTetR DNA binding domain-GBP1 fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HIR Y1158,1162,1163F

    Plasmid

    #24054

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Expression

    Mammalian

    Mutation

    Y1158F, Y1162F, Y1163F

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-Bmp7

    Plasmid

    #163591

    Purpose

    Expresses BMP-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp7 (Bmp7 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GLUT9-eGFP/pcDNA-DEST47

    Plasmid

    #18730

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

29. NavBp

    Plasmid

    #100014

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

    Depositor

    David Clapham, Paul DeCaen

    Article

    DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

    Insert

    NavBp
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV Flag cycT2b (P#1718)

    Plasmid

    #14599

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A137T compared to NP_490595.1

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lentiviral-FKBP-cpGFP-Nb39

    Plasmid

    #191498

    Purpose

    Rapamycin-dependent opioid sensor for opioid receptors (component 2)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    FKBP-cpGFP-Nb39
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-ChroME(S273A)-mRuby2-ST

    Plasmid

    #109128

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(S273A)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGFH-9

    Plasmid

    #39538

    Depositor

    Katharina Strub

    Article

    Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

    Insert

    SRP9 (SRP9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEntr1a-wGBD-mTurquoise2-wGBD

    Plasmid

    #176136

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Entry vector

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSG5-hSyntenin2a

    Plasmid

    #89432

    Purpose

    mammalian expression of human Syntenin2a

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHBintN

    Plasmid

    #48135

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pRRL-GFPGO2(NGG)-PGK-Neo

    Plasmid

    #136891

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2(NGG)
    

    Use

    Lentiviral

    Tags

    2xNLS

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge