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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 17,910 results for: REV

Showing: 9251 - 9300 of 17910 results

  11. pcDNA3-ER-AKAR4

    Plasmid
    #64733
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    AKAR4
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64733-plasmid-map-sequence-id-109352

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  14. pYFP-NEAT1_IS_RT

    Plasmid
    #97091
    Purpose
    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1_IS_RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97091-plasmid-map-sequence-id-189691

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  15. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Insert
    InPAkt
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  17. pJAK080

    Plasmid
    #167279
    Purpose
    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile
    Depositor
    Robert Fagan
    Article
    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11
    Type
    Empty backbone
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167279-plasmid-map-sequence-id-330742

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  19. pGMCtKq28-TSC10M1-sspB

    Plasmid
    #49526
    Purpose
    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    TSC10M1-sspB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49526-plasmid-map-sequence-id-73918

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  25. VinTS-tCRMod-GGSGGS5

    Plasmid
    #111763
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111763-plasmid-map-sequence-id-214717

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  34. PE_CO-Mini-C

    Plasmid
    #190337
    Purpose
    Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-C, PCSK9 +1A insertion pegRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    190337-plasmid-map-sequence-id-374556

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  40. pL0_AC-CEN_ARS_HIS

    Plasmid
    #202129
    Purpose
    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    CEN_ARS_HIS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ARS sequence mutated to remove sapI site for dome…
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202129-plasmid-map-sequence-id-405565

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  42. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

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Showing: 9251 - 9300 of 17910 results