167,785 results

1. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor14-ShHELIX-KanR (CJT70)

   Plasmid

   #181793

   Purpose

   pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Vimentin-C-18

   Plasmid

   #54300

   Purpose

   Localization: Intermediate Filaments, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCk2

   Plasmid

   #136696

   Purpose

   Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Ccdc134-IRES-BFP

   Plasmid

   #117266

   Purpose

   Retroviral overexpression of Ccdc134

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Ccdc134 (Ccdc134 )
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet1_His-TEV-EGFP

   Plasmid

   #223723

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   EGFP, not monomeric (has A207)
   

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

   Plasmid

   #220717

   Purpose

   AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC-HIS6-NUSA-TEV-GG

   Plasmid

   #215798

   Purpose

   Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mCherry-APEX1

   Plasmid

   #215126

   Purpose

   Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCBP1_pLX307

    Plasmid

    #98362

    Purpose

    Lentiviral expression of PCBP1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    PCBP1 (PCBP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG sHRPa-NRX

    Plasmid

    #73143

    Purpose

    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-NRX
    

    Tags

    2x HA tag and Flag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-HA C/EBP gamma

    Plasmid

    #15715

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    C/EBP gamma (CEBPG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. CAG sHRPb-NLG

    Plasmid

    #73144

    Purpose

    Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-NLG (Nlgn1 Synthetic, Mouse)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-RFP

    Plasmid

    #22910

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Insert

    Chicken Beta-actin promoter driving DsRedExpress
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    David Derse, Dmitriy Mazurov

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Promoter

    CMV/R/U5

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3472

    Plasmid

    #144948

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC3 (NFATC3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPLICS PM-ER Long P2A

    Plasmid

    #164111

    Purpose

    Detect the long Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK436 (mAID-mCherry-P2A-Hygro)

    Plasmid

    #168231

    Purpose

    C-terminal tagging with mAID-mCherry

    Depositor

    Masato Kanemaki

    Article

    Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

    Insert

    mAID-mCherry-P2A-Hygro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-GFAP-iGABASnFR2-WPRE (AAV5)

    Viral Prep

    #218871-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-GFAP-iGABASnFR2-WPRE (#218871). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Promoter

    GFAP

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pSYC-437

    Plasmid

    #163829

    Purpose

    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

    Depositor

    Seok-Yong Choi

    Article

    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

    Inserts

    Foxj1a Promoter
    GCaMP6s
    

    Use

    Multisite gateway destination vector

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. FLAG-Stx17

    Plasmid

    #45911

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAIP-hXRCC1pd-eGFP

    Plasmid

    #206033

    Purpose

    Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    CAG

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPB CAG SMARCA4-IRES-EGFP

    Plasmid

    #153948

    Purpose

    piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. mCherry-CD36-C-10

    Plasmid

    #55011

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pORF2

    Plasmid

    #206026

    Purpose

    An expression vector for mouse LINE1 ORF2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF2 derived from mouse LINE1
    

    Expression

    Mammalian

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSC142

    Plasmid

    #122835

    Purpose

    SUC2 promoter with GreenGate A-B flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    SUC2 promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. mscv2.2-6mycFlaA

    Plasmid

    #60203

    Purpose

    6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    FlaA
    

    Use

    Retroviral

    Tags

    6xMYC

    Expression

    Mammalian

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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