23,444 results

1. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

   Plasmid

   #44028

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P402A, P577A, N813A

   Promoter

   CMV

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAID-EF1a linearizing in pX330

   Plasmid

   #140610

   Purpose

   Crispr/Cas9 plasmid for linearization of pAID-EF1a

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   sgRNA for pAID-EF1a plasmid digestion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHyo245

   Plasmid

   #173147

   Purpose

   Expresses pheS_A294G by dual T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

   Insert

   pheS_A294G
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCMV4-3 HA/IkB-alpha(SS32,36AA)

   Plasmid

   #24143

   Depositor

   Warner Greene

   Article

   Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

   Insert

   IkB alpha (NFKBIA Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   S32A S36A

   Promoter

   Available since

   March 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223_KRAS_WT

   Plasmid

   #81923

   Purpose

   Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. integrin beta6 EGFP-N3

   Plasmid

   #13593

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 (ITGB6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. integrin beta6 EGFP-N3

   Plasmid

   #13593

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 (ITGB6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBtet-GB

    Plasmid

    #60504

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-HA-hUBE1L

    Plasmid

    #12438

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    UBE1L (UBA7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-HA-hUBE1L

    Plasmid

    #12438

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    UBE1L (UBA7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pHAGE-PIK3CA-E542K

    Plasmid

    #116479

    Purpose

    Lentiviral expression of PIK3CA E542K

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E542K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTurquoise2-pBAD

    Plasmid

    #54844

    Purpose

    Localization: Protein Expression Vector , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    6x His, T7 tag, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Loren Looger, GENIE Project, Douglas Kim

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. phMYT1L-N106

    Plasmid

    #60861

    Purpose

    Expresses MYT1L under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDHblast MCSNard OST-LMNAd50

    Plasmid

    #22662

    Depositor

    Tom Misteli

    Article

    Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

    Insert

    LaminAd50 (=Progerin)
    

    Use

    Lentiviral

    Tags

    Onestrep Tag

    Expression

    Mammalian

    Mutation

    50AA deletion creating Progerin instead of mature…

    Promoter

    Available since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc E. coli, Fly)
    

    Use

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Promoter

    Available since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
    

    Use

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Promoter

    Available since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Fibronectin-human-plasma in pMAX

    Plasmid

    #120401

    Purpose

    enables eukaryotic expression of human plasma fibronectin

    Depositor

    Inaam Nakchbandi

    Article

    Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

    Insert

    Fibronectin (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBRIT Pax7 FLAG

    Plasmid

    #17521

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pBRIT Pax7 FLAG

    Plasmid

    #17521

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pCMV-Caspase1-flag

    Plasmid

    #21142

    Depositor

    Junying Yuan

    Article

    Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

    Insert

    caspase-1 (Casp1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Human Lentiviral sgRNA Library - Nuclear

    Pooled Library

    #51047

    Purpose

    Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hSERT pcDNA3

    Plasmid

    #15483

    Depositor

    Randy Blakely

    Article

    Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

    Insert

    hSERT (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hSERT pcDNA3

    Plasmid

    #15483

    Depositor

    Randy Blakely

    Article

    Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

    Insert

    hSERT (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid

    #188902

    Purpose

    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-KRAB(Kox1)-MeCP2
    

    Use

    Lentiviral

    Tags

    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET32a-HD25Q

    Plasmid

    #11508

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 25 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 25 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET32a-HD25Q

    Plasmid

    #11508

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 25 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 25 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mTagRFP-Membrane-1

    Plasmid

    #57992

    Purpose

    Localization: Plasma Membrane, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    N-terminal 20 Aas MLS from Neuromodulin and mTagR…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTet-O-ATOH1-T2A-PuroR

    Plasmid

    #162342

    Purpose

    Lentiviral expression of ATOH1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

    Plasmid

    #55640

    Purpose

    Dre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

    Plasmid

    #99608

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    iCRE
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pENTR.CMV

    Plasmid

    #32688

    Purpose

    Mammalian expression and gateway entry vector

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. DRH337: AAV-hsyn-CheRiff-eGFP

    Plasmid

    #51697

    Purpose

    AAV production of CheRiff channelrhodopsin actuator

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    CheRiff-eGFP
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. p3E_BAG3wt-eGFP

    Plasmid

    #98180

    Purpose

    Codes for full length, GFP tagged, human wildtype Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3wt-eGFP (BAG3 Human)
    

    Use

    Gateway vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBT128c

    Plasmid

    #138512

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Use

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. GFP-BioID-FAM21

    Plasmid

    #121046

    Purpose

    Expresses GFP tagged BioID and human FAM21C in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Inserts

    BioID
    FAM21C (FAM21C Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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