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Showing: 571 - 600 of 2660 results

  2. pTC-CMV-Tet-YAP

    Plasmid
    #85579
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Inserts
    CreER
    YAP
    Use
    Cre/Lox
    Tags
    Flag (2x)
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85579-plasmid-map-sequence-id-169835

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  3. pCW57-CMV-MAAP-SL01V6

    Plasmid
    #218249
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218249-plasmid-map-sequence-id-449746

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  4. pCW57-CMV-MAAP-SL01V9

    Plasmid
    #218250
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218250-plasmid-map-sequence-id-449681

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  14. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

    Plasmid
    #204151
    Purpose
    Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Insert
    SARS-CoV-2 Delta variant spike
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    TRE3G
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204151-plasmid-map-sequence-id-404782

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  15. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  16. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  20. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  25. EW488 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(T2098L)

    Plasmid
    #236159
    Purpose
    Express NZF transcriptional activation domain fused to a deimmunized linker and mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    NZF-deImmunLink-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236159-plasmid-map-sequence-id-468430

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  30. pCMV sMTase (eGFP)

    Plasmid
    #89930
    Purpose
    Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    dCas9-MC-IRES-MN
    Use
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    deactivated Cas9
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89930-plasmid-map-sequence-id-178000

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Showing: 571 - 600 of 2660 results