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169,450 results

Showing: 6001 - 6030 of 169450 results
  1. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Insert
    humanized denOsCas12f1-VPR
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. MSCV h c-MYC IRES GFP

    Plasmid
    #18119
    Depositor
    Insert
    c-MYC (MYC Human)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    The CUG which is the start site for Myc I is pres…
    Available Since
    June 9, 2008
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-CamKII-ArchT-GFP (PV2527) (AAV5)

    Viral Prep
    #99039-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition.
    Depositor
    Promoter
    CaMKII
    Tags
    GFP
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available Since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 6001 - 6030 of 169450 results