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175,750 results

Showing: 8041 - 8080 of 175750 results

  1. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid
    #205703
    Purpose
    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines
    Depositor
    Kai Johnsson
    Article
    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.
    Insert
    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    205703-plasmid-map-sequence-id-423137

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  2. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    83307-plasmid-map-sequence-id-173284

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  5. pBItetDT960GFP

    Plasmid
    #80419
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80419-plasmid-map-sequence-id-495965

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  9. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  14. IDG_SCN2B_OE_1

    Plasmid
    #161624
    Purpose
    Overexpresses SCN2B
    Depositor
    Mike McManus
    Insert
    SCN2B (SCN2B Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    161624-plasmid-map-sequence-id-334519

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  19. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  20. pCXLE_hePOU_shp53

    Plasmid
    #206397
    Purpose
    Integration-free (episomal) expression of human ePOU and shRNA against p53.
    Depositor
    Ralf Jauch
    Inserts
    ePOU
    shRNA against p53 (TP53 Human)
    Expression
    Mammalian
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206397-plasmid-map-sequence-id-409986

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  28. pDest-566

    Plasmid
    #11517
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11517-plasmid-map-sequence-id-299808

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  29. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  30. dCas9-GCN5 FL

    Plasmid
    #179552
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179552-plasmid-map-sequence-id-354751

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  31. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid
    #50478
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50478-plasmid-map-sequence-id-166302

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  34. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral Prep
    #162381-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  39. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid
    #105543
    Purpose
    AAV expression of EGFP from cTNT promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    cTNT-PI-EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    cTNT
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105543-plasmid-map-sequence-id-202437

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Showing: 8041 - 8080 of 175750 results