261,409 results

1. pET-22b(+) pCopA RBS30 mscarlet

   Plasmid

   #226370

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   mScarlet
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Promoter

   copA promoter (pCopA)

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Self-targeting epegRNA library, +5 G>H (lDS004)

   Pooled Library

   #227707

   Purpose

   CRISPR guide RNA library for prime editing

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

3. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

   Plasmid

   #221352

   Purpose

   Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC

   Plasmid

   #221351

   Purpose

   Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR221-ATP6V0D1

   Plasmid

   #166154

   Purpose

   Gateway entry vector for N. furzeri ATP6V0D1V

   Depositor

   Daniel Jarosz

   Article

   Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

   Insert

   ATP6V0D1V
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

   Plasmid

   #191737

   Purpose

   AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221-kcnk18

   Plasmid

   #209000

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk18 (kcnk18 Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

   Plasmid

   #198450

   Purpose

   Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mc2 mCherry hPGK

   Plasmid

   #206231

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF3455

    Plasmid

    #144931

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF513 (ZNF513 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF3455

    Plasmid

    #144931

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF513 (ZNF513 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TT lux sensor

    Plasmid

    #199792

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TT lux Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0088

    Plasmid

    #142455

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF408 (ZNF408 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF0088

    Plasmid

    #142455

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF408 (ZNF408 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CROP_C9_Puro_TOP2B

    Plasmid

    #183325

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROP_C9_Puro_TOP2A

    Plasmid

    #183324

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid

    #140271

    Purpose

    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid

    #140271

    Purpose

    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGDP1 stat

    Plasmid

    #112886

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    stat
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGDP1 stat

    Plasmid

    #112886

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    stat
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-hSyn-Soma-jGCaMP8s (AAV9)

    Viral Prep

    #169256-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Promoter

    Tags

    None

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

24. pVC-FUS-R521C

    Plasmid

    #175067

    Purpose

    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    changed Arginin 521 to Cystein; mVenus: C-Termina…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EF.STAT3DN.Ubc.GFP

    Plasmid

    #24984

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F--dominant negative

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF.STAT3DN.Ubc.GFP

    Plasmid

    #24984

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F--dominant negative

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDN34-BiNLS-hYAP1-1delta

    Plasmid

    #169471

    Purpose

    expresses optoYAP in mammalian cells

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    YAP1-1delta (YAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Actin-cpstFRET-Actin

    Plasmid

    #80643

    Purpose

    cpstFRET actin sensor for mechanical force measurements in cytoskeleton

    Depositor

    Fred Sachs

    Article

    Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

    Insert

    Beta actin, cpstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Actin-cpstFRET-Actin

    Plasmid

    #80643

    Purpose

    cpstFRET actin sensor for mechanical force measurements in cytoskeleton

    Depositor

    Fred Sachs

    Article

    Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

    Insert

    Beta actin, cpstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCCL/IFNB1-d2eGFP-3’UTR

    Plasmid

    #180232

    Purpose

    Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

    Insert

    d2eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1 kb upstream of IFNB1 CDS

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mScarlet-EEA1

    Plasmid

    #169067

    Purpose

    Express human EEA1 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH-EF1-copGFP-T2A-Puro

    Plasmid

    #72263

    Purpose

    Express copGFP and puromycin resistance gene under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCDH-EF1-copGFP-T2A-Puro

    Plasmid

    #72263

    Purpose

    Express copGFP and puromycin resistance gene under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPPAT-EGFP

    Plasmid

    #99105

    Purpose

    Expresses human PPAT-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPPAT-EGFP

    Plasmid

    #99105

    Purpose

    Expresses human PPAT-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge