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232,742 results

Showing: 10151 - 10200 of 232742 results

  1. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

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  2. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

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  3. p006-Banana-Late

    Plasmid
    #112251
    Purpose
    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    ATF1 under osmY promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    osmY
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112251-plasmid-map-sequence-id-218818

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  4. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  9. pLenti6.2-Nanoluc-ccdB

    Plasmid
    #87078
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87078-plasmid-map-sequence-id-173606

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  10. pLenti6.2-Nanoluc-ccdB

    Plasmid
    #87078
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87078-plasmid-map-sequence-id-173606

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  13. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Promoter
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

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  14. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid
    #225755
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225755-plasmid-map-sequence-id-446065

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  17. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  20. OMM-SPLICSs-nFAST

    Plasmid
    #210430
    Purpose
    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    OMM-SPLICSs-nFAST
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210430-plasmid-map-sequence-id-415625

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  27. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

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  28. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

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  32. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  36. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  37. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  42. pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)

    Viral Prep
    #52473-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-synP-FLEX-splitTVA-EGFP-B19G (#52473). In addition to the viral particles, you will also receive purified pAAV-synP-FLEX-splitTVA-EGFP-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Article
    Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  43. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  44. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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Showing: 10151 - 10200 of 232742 results