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178,238 results

Showing: 10151 - 10200 of 178238 results

  1. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  6. pAAV-CaMKIIa-mCherry (AAV2)

    Viral Prep
    #114469-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-hsyn-GRAB_DA-mut (AAV9)

    Viral Prep
    #140555-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA-mut (#140555). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA-mut plasmid DNA. Synapsin-driven expression of control dopamine sensor GRAB_DA-mut (does not respond to DA). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  9. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  11. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

    Viral Prep
    #137130-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    BFP (Cre-dependent)
    Available Since
    March 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  25. pCAGGs-RFP

    Plasmid
    #127347
    Purpose
    Expresses RFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Red Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CAGGS Promoter
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127347-plasmid-map-sequence-id-246568

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  27. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    MultiSite Gateway
    Available Since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  34. pLenti-EB1-EGFP

    Plasmid
    #118084
    Purpose
    Expresses EB1-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human EB1 (MAPRE1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118084-plasmid-map-sequence-id-230385

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  35. pGTMV

    Plasmid
    #118755
    Purpose
    Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118755-plasmid-map-sequence-id-229554

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  36. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117006-plasmid-map-sequence-id-227036

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  40. pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV8)

    Viral Prep
    #105542-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA. CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CB7
    Tags
    EGFP
    Available Since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  50. pAAV.hSyn.iGluSnFr.WPRE.SV40 (AAV5)

    Viral Prep
    #98929-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.hSyn.iGluSnFr.WPRE.SV40 (#98929). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFr.WPRE.SV40 plasmid DNA. Synapsin-driven, iGluSnFr glutamate sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Promoter
    Syn
    Available Since
    Feb. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 10151 - 10200 of 178238 results