We narrowed to 34,877 results for: cas

1. CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

   Plasmid

   #138419

   Purpose

   CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BioTAP-tag

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200631

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTRE3G

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

   Plasmid

   #100596

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-gRNA(anti-cGAS)-Cas9-Puro

   Plasmid

   #130908

   Purpose

   gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118155

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

   Plasmid

   #117405

   Purpose

   SpyCas9 sgRNA 1 targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SpyCas9 sgRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177345

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Synapsin

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Use

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gal1/10p CAS9 Gal1/10p I-SceI

    Plasmid

    #182519

    Purpose

    Galactose-Induced Expression vector for Cas9 and I-SceI.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Inserts

    I-SceI
    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-NES-AdmCas13d-msfGFP-NES-Flag

    Plasmid

    #165075

    Purpose

    overexpression of AdmCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    AdmCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

    Plasmid

    #113039

    Purpose

    AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid

    #196086

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting lncRNA NORAD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

    Plasmid

    #158987

    Purpose

    Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP160
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

    Plasmid

    #86987

    Purpose

    For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid

    #216733

    Purpose

    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase and sgCTG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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