167,825 results

1. ABE-NW1

   Plasmid

   #240615

   Purpose

   Adenine base editor with narrowed editing window

   Depositor

   Tingting Jiang

   Article

   Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

   Insert

   TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

   Available Since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAP2K2-NanoLuc Fusion Vector

   Plasmid

   #238877

   Purpose

   Express MAP2K2-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. HAP40 1-371

   Plasmid

   #124060

   Purpose

   Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HAP40 (F8A1 Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Promoter

   polyhedrin

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

5. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMUH_unc84_2XGFP

   Plasmid

   #46023

   Depositor

   Sean Eddy

   Article

   Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

   Insert

   UNC-84 (unc-84 )
   

   Use

   10xuas-phi31-expression vector

   Tags

   2XGFP

   Mutation

   Two amino acid mutations (F279L and L500P) compar…

   Promoter

   10XUAS

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3-EF1a-NICD1

   Plasmid

   #46047

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain initiating at amino acid 1753

   Promoter

   EF1a

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcSTH-LbNOX

   Plasmid

   #211924

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH-LbNOX
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113611

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    mu opioid receptor
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid

    #154827

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    TagBFP
    HygR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-Cdk5

    Plasmid

    #24894

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    CDK5 (CDK5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-HA-UCHL1

    Plasmid

    #22563

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    UCHL1 (UCHL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYO395

    Plasmid

    #235734

    Purpose

    Expression of human VPS34 complex I in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Inserts

    ATG14 (ATG14 Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Tags

    ZZ-3xTEV

    Expression

    Mammalian

    Mutation

    N256S

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. DOP1B_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176979

    Purpose

    Mammalian expression vector encoding DOP1B and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    DOP1B (DOPEY2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-BbTagBY (AAV9)

    Viral Prep

    #45185-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Joshua Sanes, Dawen Cai

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Promoter

    EF1a

    Tags

    TagBFP, EYFP, or none.

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221-NPC1

    Plasmid

    #132295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pLentiCMV-Blast

    Plasmid

    #125133

    Purpose

    Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP_WT-talin1 (1-2541)

    Plasmid

    #166112

    Purpose

    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    WT-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET His10 TEV LIC cloning vector (2B-T-10)

    Plasmid

    #78173

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    10xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. EXT1-pLJC2-3XFLAG

    Plasmid

    #215818

    Purpose

    Ectopic expression of EXT-1 in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBABEpuro-ERBB2 A775_G776insYVMA

    Plasmid

    #40982

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A775_G776insYVMA

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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29. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV MP-135 mRFP

    Plasmid

    #206927

    Purpose

    The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-135

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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