We narrowed to 10,362 results for: Coli
-
Plasmid#224251PurposeExpresses human KDAC4 (HDAC4) catalytic domain in E. coliDepositorInsertKDAC4 (HDAC4 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC7 CD
Plasmid#224341PurposeExpresses human KDAC7 (HDAC7) catalytic domain in E. coliDepositorInsertKDAC7 (HDAC7 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 521-942 (catalytic domain).PromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU1-A-pdh_RiboJ_mCherry_Bba_B0015
Plasmid#107581PurposeB. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialPromoterpdh promoter Bacillus megaterium DSM319Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
Plasmid#69138PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorInsertCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
TagsnoneExpressionMammalianAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
Plasmid#160540PurposeExpresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.DepositorInsertsExpressionBacterialAvailable SinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF1-4X
Plasmid#120282PurposeProduce human recombinant FGF1-4X in DE3 cellsDepositorInsertBasic fibroblast growth factor 1 mutant
Tags6x His tagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
TagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1_RAF1 (51-131)
Plasmid#119218Purposepurification of GST-RAF1 RBD from E. coliDepositorInsertRAF1 aa 51-131 (RAF1 Human)
ExpressionBacterialAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
esmGFP-C10-pVTCB052
Plasmid#231303PurposePlasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coliDepositorInsertesmGFP-C10
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.1.t1
Plasmid#73545PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.1.t1
pAcFRS.1.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-p53 (1-90) (72R)
Plasmid#62081PurposeExpression of human p53 (TAD residues 1-90) (72R) in e. coliDepositorInsertp53 (TP53 Human)
TagsGSTExpressionBacterialMutationContains TAD/PP residues 1-90, 72RPromotertacAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRExpressionMammalianAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SET
Plasmid#234708Purposeexpresses His-tagged human SET in E coliDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43
Plasmid#134574PurposeExpress human DDX43 protein (WT, full-length) in E. coliDepositorAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
OPEN-HLA-B*07:02-BirA
Plasmid#239752PurposeE. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulinDepositorInsertOPEN-HLA-B*07:02 (HLA-B Human)
TagsBirA substrate peptideExpressionBacterialMutationG120CPromoterT7Available SinceAug. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA(D34A/D169A)
Plasmid#108693PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon, …PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_LC3A
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
TagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialPromotertac promoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
Tags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
R701-X71-527: His6-tev-Hs.CALM1(1-80)
Plasmid#159694PurposeE. coli protein expression of His6-tev-Hs.CALM1(1-80)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
Plasmid#154881PurposeDoxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorInsertsExpressionMammalianAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
R701-X72-527: His6-tev-Hs.CALM1(76-148)
Plasmid#159695PurposeE. coli protein expression of His6-tev-Hs.CALM1(76-148)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)Available SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse LaminA
Plasmid#182674PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.DepositorInsertLamin A (Lmna Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianPromoterHSPAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (23-111)
Plasmid#62064PurposeExpression of human MDM4 (residues 23-111) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 23-111PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GEC1
Plasmid#73945PurposeFor recombinant expression of human GEC1/GABARAPL1 in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (1-120)
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
KRAS-P34R
Plasmid#158527PurposeExpresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-90) (72P)
Plasmid#62080PurposeExpression of human p53 (1-90) (72P) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD/PP residues 1-90; 72PPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
TagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only