We narrowed to 14,002 results for: flag

1. pCMV-dLight1.1

   Plasmid

   #111053

   Purpose

   Expresses dLight1.1 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.1
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NMUR2-Tango

   Plasmid

   #66447

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   NMUR2 (NMUR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Lenti-Dual-iCas9-hALB

   Plasmid

   #167502

   Purpose

   Expression of dual inducible Cas9 with hALB promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hALB

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FFA2-Tango

   Plasmid

   #66281

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FFA2 (FFAR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FPR1-Tango

   Plasmid

   #66283

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR1 (FPR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. S1PR2-Tango

   Plasmid

   #66497

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   S1PR2 (S1PR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FPR3-Tango

   Plasmid

   #66285

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR3 (FPR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-dLight1.3b

    Plasmid

    #111056

    Purpose

    Expresses dLight1.3b in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3b
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSynapsin1-RdLight1

    Plasmid

    #125708

    Purpose

    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Has Service

    AAV9

    Insert

    RdLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIRES-hrGFP II-TET1

    Plasmid

    #83568

    Purpose

    Expresses TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FFA3-Tango

    Plasmid

    #66282

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    FFA3 (FFAR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-RdLight1

    Plasmid

    #125707

    Purpose

    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-dLight1.2

    Plasmid

    #111054

    Purpose

    Expresses dLight1.2 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.2
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GPR44-Tango

    Plasmid

    #66359

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR44 (PTGDR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Lenti-Dual-iCas9-EFS

    Plasmid

    #167501

    Purpose

    Expression of dual inducible Cas9 with EFS promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1_rSERT_WT

    Plasmid

    #190743

    Purpose

    For expression of wild-type rat SERT in mammalian cells

    Depositor

    Gary Rudnick

    Article

    Singh et al bioRxiv 2022.06.13.495991

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-STAG2-wildtype

    Plasmid

    #31972

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLpA-DN.JNK1

    Plasmid

    #51942

    Purpose

    Adenoviral vector for dominant negative JNK1 expression

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK1 (Mapk8 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

    Plasmid

    #174899

    Purpose

    Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Use

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

28. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTXB1-TP5

    Plasmid

    #160088

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTXB1-TP3

    Plasmid

    #160086

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSIN4-EF1a-TAL1-IRES-Puro

    Plasmid

    #61065

    Purpose

    lentiviral vector for TAL1

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    TAL1 (TAL1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. plexm-human LRRC33

    Plasmid

    #111600

    Purpose

    Express human LRRC33 in 393T cells

    Depositor

    Timothy Springer

    Article

    Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

    Insert

    LRRC33 (NRROS Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCV-human-pro-IL-18

    Plasmid

    #214317

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. S1PR1-Tango

    Plasmid

    #66496

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    S1PR1 (S1PR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYPQ166-OsPE2

    Plasmid

    #141080

    Purpose

    Gateway entry clone for CRISPR-Cas9(H840A) prime editing

    Depositor

    Yiping Qi

    Article

    Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

    Insert

    zCas9(H840A)-OsM-MLV-RT
    

    Use

    CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid

    #89637

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. S1PR3-Tango

    Plasmid

    #66498

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    S1PR3 (S1PR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pENTR221-YAP1

    Plasmid

    #79503

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GPR141-Tango

    Plasmid

    #66318

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR141 (GPR141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. GPRC5D-Tango

    Plasmid

    #66385

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC5D (GPRC5D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pEBTet-SNAP-CP110

    Plasmid

    #136802

    Purpose

    Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CP110 (CCP110 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGWB412

    Plasmid

    #74806

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3 merlin 1-595

    Plasmid

    #11623

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only