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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,308 results for: ENA

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Showing: 10651 - 10700 of 11308 results

  5. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    enhancer from 7SL gene
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

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  7. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  8. LV-Let7d-5p

    Plasmid
    #117055
    Purpose
    Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression
    Depositor
    Michele De Palma
    Article
    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d
    Insert
    Let-7d-5p - OFP
    Use
    Lentiviral
    Promoter
    SFFV promoter
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117055-plasmid-map-sequence-id-223998

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  15. pYPQ-SpRY-Act3.0

    Plasmid
    #178957
    Purpose
    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178957-plasmid-map-sequence-id-354306

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  23. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  31. pCI-TPI_PTC160-xrRNA-4H

    Plasmid
    #108369
    Purpose
    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Mutation
    PTC160
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108369-plasmid-map-sequence-id-209440

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  37. pFAB1935

    Plasmid
    #47860
    Purpose
    BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    his_T_min_linker_crp_T_min double terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    concatenate of his_min and crp_min
    Promoter
    pLTetO15
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47860-plasmid-map-sequence-id-68392

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  39. R4pGWB401+Citrine

    Plasmid
    #128432
    Purpose
    Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.
    Depositor
    George Haughn
    Article
    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Citrine, yellow fluorescence protein
    Expression
    Plant
    Available Since
    Oct. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    128432-plasmid-map-sequence-id-257650

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  40. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  47. pFAB1902

    Plasmid
    #47858
    Purpose
    BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency
    Depositor
    Drew Endy
    Article
    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:
    Insert
    M13_central_T_linker_rrnD_T1 double terminator
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    concatenate of M13 central and rrnD
    Promoter
    pLTetO13
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47858-plasmid-map-sequence-id-68390

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  48. pTARGEX-VAC

    Plasmid
    #242450
    Purpose
    Targets high levels of recombinant protein to vacuole. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    C-terminal propeptide (CTPP) derived from N. taba…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242450-plasmid-map-sequence-id-489544

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  50. FLuc-C-HiBiT

    Plasmid
    #207118
    Purpose
    Bacterially expressed FLuc-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207118-plasmid-map-sequence-id-417161

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Showing: 10651 - 10700 of 11308 results