Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

165,803 results

Showing: 8641 - 8680 of 165803 results

  1. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

    Enlarge

  10. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

    Enlarge

  12. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (AAV9)

    Viral Prep
    #105556-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (#105556). In addition to the viral particles, you will also receive purified pENN.AAV.tMCK.PI.eGFP.WPRE.bGH plasmid DNA. Expression of EGFP from tMCK promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    tMCK
    Tags
    EGFP
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pShuttle

    Plasmid
    #16402
    Purpose
    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    16402-plasmid-map-sequence-id-237978

    Enlarge

  21. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

    Viral Prep
    #51084-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV-hSyn-GRAB_OT1.0 (AAV9)

    Viral Prep
    #185386-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  23. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

    Enlarge

  25. pLIX403-EYFP-ccdB-Zeo

    Plasmid
    #158565
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158565-plasmid-map-sequence-id-309880

    Enlarge

  27. pAAV-CAG-dLight1.3b (AAV1)

    Viral Prep
    #125560-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    CAG
    Tags
    None
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  28. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

    Enlarge

  32. pGBW-m4134902

    Plasmid
    #151912
    Purpose
    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151912-plasmid-map-sequence-id-307617

    Enlarge

  33. pJCX4

    Plasmid
    #170756
    Purpose
    Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    Mxe GyrA intein - chitin binding domain (CBD)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170756-plasmid-map-sequence-id-334719

    Enlarge

  34. pAAV-FLEX-GFP

    Plasmid
    #28304
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available Since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28304-plasmid-map-sequence-id-454424

    Enlarge

  35. GPR27-Tango

    Plasmid
    #66349
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR27 (GPR27 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66349-plasmid-map-sequence-id-456518

    Enlarge

  37. pOD2045-intControl

    Plasmid
    #100005
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100005-plasmid-map-sequence-id-193781

    Enlarge

  39. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)

    Viral Prep
    #104491-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available Since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 8641 - 8680 of 165803 results